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Literature summary extracted from

  • Liu, M.; Douthwaite, S.
    Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes gram-negative from gram-positive bacteria (2002), Mol. Microbiol., 44, 195-204.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.187
-
 Shewanella putrefaciens
2.1.1.187
-
 Acinetobacter sp.
2.1.1.187
-
 Escherichia coli
2.1.1.188
-
 Bacillus subtilis
2.1.1.188
-
 Streptococcus pneumoniae
2.1.1.188
-
 Corynebacterium glutamicum
2.1.1.188
-
 Micromonospora griseorubida
2.1.1.188
-
 Streptomyces fradiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Shewanella putrefaciens Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Acinetobacter sp. Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Escherichia coli Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Azotobacter vinelandii Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Azotobacter vinelandii shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Klebsiella aerogenes Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterobacter aerogenes shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Dickeya chrysanthemi Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Erwinia crysanthemi shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Proteus mirabilis Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Proteus mirabilis shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Pseudomonas fluorescens Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas fluorescens shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Pseudomonas putida Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas putida shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Pseudomonas stutzeri Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas stutzeri shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Pseudomonas syringae Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas syringae shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Acinetobacter sp. ADP1 Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Priestia megaterium Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Bacillus megaterium shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Enterococcus faecalis Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterococcus faecalis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Lactiplantibacillus plantarum Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactobacillus plantarum shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Lactococcus lactis Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactococcus lactis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Rhodococcus corynebacterioides Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Nocardia corynebacteroides shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Streptococcus thermophilus Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Streptococcus thermophilus shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Bacillus subtilis Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Streptococcus pneumoniae Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Corynebacterium glutamicum Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Micromonospora griseorubida Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Streptomyces fradiae Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.187 Acinetobacter sp. Q9AEP4 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.187 Acinetobacter sp. ADP1 Q9AEP4 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.187 Azotobacter vinelandii C1DSW3 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.187 Dickeya chrysanthemi
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.187 Escherichia coli P36999 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.187 Klebsiella aerogenes
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.187 Proteus mirabilis
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.187 Pseudomonas fluorescens
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.187 Pseudomonas putida
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.187 Pseudomonas stutzeri A4VMZ0 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.187 Pseudomonas syringae Q48F48 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.187 Shewanella putrefaciens
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.188 Bacillus subtilis
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.188 Corynebacterium glutamicum
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.188 Enterococcus faecalis
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.188 Lactiplantibacillus plantarum
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.188 Lactococcus lactis
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.188 Micromonospora griseorubida
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.188 Priestia megaterium
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.188 Rhodococcus corynebacterioides
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.188 Streptococcus pneumoniae
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.188 Streptococcus thermophilus
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
2.1.1.188 Streptomyces fradiae Q9S1M6 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.187
-
 Shewanella putrefaciens
2.1.1.187
-
 Acinetobacter sp.
2.1.1.187
-
 Escherichia coli
2.1.1.188
-
 Bacillus subtilis
2.1.1.188
-
 Streptococcus pneumoniae
2.1.1.188
-
 Corynebacterium glutamicum
2.1.1.188
-
 Micromonospora griseorubida
2.1.1.188
-
 Streptomyces fradiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Shewanella putrefaciens S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Acinetobacter sp. S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Azotobacter vinelandii shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Azotobacter vinelandii S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterobacter aerogenes shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Klebsiella aerogenes S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Erwinia crysanthemi shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Dickeya chrysanthemi S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Proteus mirabilis shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Proteus mirabilis S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas fluorescens shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Pseudomonas fluorescens S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas putida shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Pseudomonas putida S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas stutzeri shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Pseudomonas stutzeri S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas syringae shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Pseudomonas syringae S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.187 S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Acinetobacter sp. ADP1 S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Bacillus megaterium shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro Priestia megaterium S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterococcus faecalis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro Enterococcus faecalis S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactobacillus plantarum shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro Lactiplantibacillus plantarum S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactococcus lactis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro Lactococcus lactis S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Nocardia corynebacteroides shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro Rhodococcus corynebacterioides S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Streptococcus thermophilus shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro Streptococcus thermophilus S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Bacillus subtilis S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Streptococcus pneumoniae S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Corynebacterium glutamicum S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Micromonospora griseorubida S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Streptomyces fradiae S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.187 RlmA ambiguous Escherichia coli
2.1.1.187 RlmAI
-
 Pseudomonas fluorescens
2.1.1.187 RlmAI
-
 Klebsiella aerogenes
2.1.1.187 RlmAI
-
 Pseudomonas putida
2.1.1.187 RlmAI
-
 Proteus mirabilis
2.1.1.187 RlmAI
-
 Dickeya chrysanthemi
2.1.1.187 RlmAI
-
 Shewanella putrefaciens
2.1.1.187 RlmAI
-
 Azotobacter vinelandii
2.1.1.187 RlmAI
-
 Pseudomonas stutzeri
2.1.1.187 RlmAI
-
 Pseudomonas syringae
2.1.1.187 RlmAI
-
 Acinetobacter sp.
2.1.1.187 RlmAI
-
 Escherichia coli
2.1.1.187 rRNA large subunit methyltransferase I
-
 Pseudomonas fluorescens
2.1.1.187 rRNA large subunit methyltransferase I
-
 Klebsiella aerogenes
2.1.1.187 rRNA large subunit methyltransferase I
-
 Pseudomonas putida
2.1.1.187 rRNA large subunit methyltransferase I
-
 Proteus mirabilis
2.1.1.187 rRNA large subunit methyltransferase I
-
 Dickeya chrysanthemi
2.1.1.187 rRNA large subunit methyltransferase I
-
 Shewanella putrefaciens
2.1.1.187 rRNA large subunit methyltransferase I
-
 Azotobacter vinelandii
2.1.1.187 rRNA large subunit methyltransferase I
-
 Pseudomonas stutzeri
2.1.1.187 rRNA large subunit methyltransferase I
-
 Pseudomonas syringae
2.1.1.187 rRNA large subunit methyltransferase I
-
 Acinetobacter sp.
2.1.1.187 rRNA large subunit methyltransferase I
-
 Escherichia coli
2.1.1.188 RlmAII
-
 Lactiplantibacillus plantarum
2.1.1.188 RlmAII
-
 Bacillus subtilis
2.1.1.188 RlmAII
-
 Priestia megaterium
2.1.1.188 RlmAII
-
 Lactococcus lactis
2.1.1.188 RlmAII
-
 Enterococcus faecalis
2.1.1.188 RlmAII
-
 Streptococcus pneumoniae
2.1.1.188 RlmAII
-
 Streptococcus thermophilus
2.1.1.188 RlmAII
-
 Micromonospora griseorubida
2.1.1.188 RlmAII
-
 Rhodococcus corynebacterioides
2.1.1.188 RlmAII
-
 Streptomyces fradiae
2.1.1.188 rRNA large subunit methyltransferase II
-
 Lactiplantibacillus plantarum
2.1.1.188 rRNA large subunit methyltransferase II
-
 Bacillus subtilis
2.1.1.188 rRNA large subunit methyltransferase II
-
 Priestia megaterium
2.1.1.188 rRNA large subunit methyltransferase II
-
 Lactococcus lactis
2.1.1.188 rRNA large subunit methyltransferase II
-
 Enterococcus faecalis
2.1.1.188 rRNA large subunit methyltransferase II
-
 Streptococcus pneumoniae
2.1.1.188 rRNA large subunit methyltransferase II
-
 Streptococcus thermophilus
2.1.1.188 rRNA large subunit methyltransferase II
-
 Micromonospora griseorubida
2.1.1.188 rRNA large subunit methyltransferase II
-
 Rhodococcus corynebacterioides
2.1.1.188 rRNA large subunit methyltransferase II
-
 Streptomyces fradiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.187 37
-
 assay at Pseudomonas fluorescens
2.1.1.187 37
-
 assay at Klebsiella aerogenes
2.1.1.187 37
-
 assay at Pseudomonas putida
2.1.1.187 37
-
 assay at Proteus mirabilis
2.1.1.187 37
-
 assay at Dickeya chrysanthemi
2.1.1.187 37
-
 assay at Shewanella putrefaciens
2.1.1.187 37
-
 assay at Azotobacter vinelandii
2.1.1.187 37
-
 assay at Pseudomonas stutzeri
2.1.1.187 37
-
 assay at Pseudomonas syringae
2.1.1.187 37
-
 assay at Acinetobacter sp.
2.1.1.187 37
-
 assay at Escherichia coli
2.1.1.188 37
-
 assay at Lactiplantibacillus plantarum
2.1.1.188 37
-
 assay at Bacillus subtilis
2.1.1.188 37
-
 assay at Priestia megaterium
2.1.1.188 37
-
 assay at Lactococcus lactis
2.1.1.188 37
-
 assay at Enterococcus faecalis
2.1.1.188 37
-
 assay at Streptococcus pneumoniae
2.1.1.188 37
-
 assay at Corynebacterium glutamicum
2.1.1.188 37
-
 assay at Streptococcus thermophilus
2.1.1.188 37
-
 assay at Micromonospora griseorubida
2.1.1.188 37
-
 assay at Rhodococcus corynebacterioides
2.1.1.188 37
-
 assay at Streptomyces fradiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.187 7.5
-
 assay at Pseudomonas fluorescens
2.1.1.187 7.5
-
 assay at Klebsiella aerogenes
2.1.1.187 7.5
-
 assay at Pseudomonas putida
2.1.1.187 7.5
-
 assay at Proteus mirabilis
2.1.1.187 7.5
-
 assay at Dickeya chrysanthemi
2.1.1.187 7.5
-
 assay at Shewanella putrefaciens
2.1.1.187 7.5
-
 assay at Azotobacter vinelandii
2.1.1.187 7.5
-
 assay at Pseudomonas stutzeri
2.1.1.187 7.5
-
 assay at Pseudomonas syringae
2.1.1.187 7.5
-
 assay at Acinetobacter sp.
2.1.1.187 7.5
-
 assay at Escherichia coli
2.1.1.188 7.5
-
 assay at Lactiplantibacillus plantarum
2.1.1.188 7.5
-
 assay at Bacillus subtilis
2.1.1.188 7.5
-
 assay at Priestia megaterium
2.1.1.188 7.5
-
 assay at Lactococcus lactis
2.1.1.188 7.5
-
 assay at Enterococcus faecalis
2.1.1.188 7.5
-
 assay at Streptococcus pneumoniae
2.1.1.188 7.5
-
 assay at Corynebacterium glutamicum
2.1.1.188 7.5
-
 assay at Streptococcus thermophilus
2.1.1.188 7.5
-
 assay at Micromonospora griseorubida
2.1.1.188 7.5
-
 assay at Rhodococcus corynebacterioides
2.1.1.188 7.5
-
 assay at Streptomyces fradiae