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Literature summary extracted from

  • Colonna, A.; Ciliberto, G.; Santamaria, R.; Cimino, F.; Salvatore, F.
    Isolation and characterization of a tRNA(guanine-7-)-methyltransferase from Salmonella typhimurium (1983), Mol. Cell. Biochem., 52, 97-106.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.33 spermidine activates at 5 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.1.1.33 spermine activates at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.33 0.0034
-
 undermethylated tRNA
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.1.1.33 0.053
-
 S-adenosyl-L-methionine
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.33 Cations mono- and divalent cations activate Salmonella enterica subsp. enterica serovar Typhimurium
2.1.1.33 Mg2+ activates at 3 mM Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.33 S-adenosyl-L-methionine + guanine46 in tRNA Salmonella enterica subsp. enterica serovar Typhimurium tRNA modifying enzyme S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.33 Salmonella enterica subsp. enterica serovar Typhimurium
-
 wild-type
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.33
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.33 S-adenosyl-L-methionine + guanine46 in tRNA methyl group acceptor: undermethylated tRNA Salmonella enterica subsp. enterica serovar Typhimurium S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
 ?
2.1.1.33 S-adenosyl-L-methionine + guanine46 in tRNA tRNA modifying enzyme Salmonella enterica subsp. enterica serovar Typhimurium S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
 ?
2.1.1.33 S-adenosyl-L-methionine + guanine46 in undermethylated tRNA
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.33 monomer 1 * 25000-30000 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.33 S-adenosyl-L-methionine
-
 Salmonella enterica subsp. enterica serovar Typhimurium