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Literature summary extracted from

  • Jala, V.R.; Prakash, V.; Rao, N.A.; Savithri, H.S.
    Overexpression and characterization of dimeric and tetrameric forms of recombinant serine hydroxymethyltransferase from Bacillus stearothermophilus (2002), J. Biosci., 27, 233-242.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expression in Escherichia coli Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.7
-
L-serine only tetrameric form Geobacillus stearothermophilus
2.1.2.1 0.9
-
L-serine only dimeric form Geobacillus stearothermophilus
2.1.2.1 1
-
L-serine
-
Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 90000
-
gel filtration Geobacillus stearothermophilus
2.1.2.1 90000
-
dimeric form Geobacillus stearothermophilus
2.1.2.1 180000
-
tetrameric form, gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate Geobacillus stearothermophilus
-
glycine + 5,10-methylenetetrahydrofolate + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 gene cloned and expressed in Escherichia coli Geobacillus stearothermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine mechanism Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 4.1
-
only tetrameric form Geobacillus stearothermophilus
2.1.2.1 4.2
-
-
Geobacillus stearothermophilus
2.1.2.1 6.7
-
only dimeric form Geobacillus stearothermophilus
2.1.2.1 32.4
-
dimeric form at 80°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
Geobacillus stearothermophilus glycine + 5,10-methylenetetrahydrofolate + H2O
-
?
2.1.2.1 additional information enzyme catalyses the racemization of D- and L-alanine Geobacillus stearothermophilus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 More 90% dimer and 10% tetramer in the same organism, one subunit: 45000 Da, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.1 80
-
dimeric form of enzyme Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.2.1 additional information
-
Tm values of enzyme and dimeric and tetrameric forms in the absence and presence of ligands Geobacillus stearothermophilus
2.1.2.1 additional information
-
pyridoxal 5'-phosphate increases the thermal stability Geobacillus stearothermophilus
2.1.2.1 additional information
-
L-serine increases the thermal stability Geobacillus stearothermophilus
2.1.2.1 60
-
enzyme stable in the absence of any ligand Geobacillus stearothermophilus
2.1.2.1 70
-
50% loss of activity in the absence of any ligand Geobacillus stearothermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 3.6
-
L-serine only tetrameric form Geobacillus stearothermophilus
2.1.2.1 4.3
-
L-serine
-
Geobacillus stearothermophilus
2.1.2.1 5
-
L-serine only dimeric form Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
Geobacillus stearothermophilus