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Literature summary extracted from

  • Talwar, R.; Appaji Rao, N.; Savithri, H.S.
    A change in reaction specificity of sheep liver serine hydroxymethyltransferase: induction of NADH oxidation upon mutation of His230 to Tyr (2000), Eur. J. Biochem., 267, 929-934.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expression in Escherichia coli Ovis aries

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.1 H230Y 90% loss of enzyme activity, confers ability to oxidize NADH Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Ovis aries
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 additional information
-
-
 Ovis aries
2.1.2.1 0.52
-
 mutant enzyme Ovis aries
2.1.2.1 4.8
-
-
 Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Ovis aries glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 additional information enzyme catalyzes the pyridoxal 5'-phosphate dependent reversible cleavage of 3-hydroxy-alpha-amino acids Ovis aries ?
-
 ?
2.1.2.1 additional information mutant enzyme H230Y catalyses oxidation of NADH, not wild type enzyme, H230A, H230F, H230N Ovis aries ?
-
 ?
2.1.2.1 additional information enzyme transaminates D-alanine to pyruvate and pyridoxamine phosphate Ovis aries ?
-
 ?
2.1.2.1 additional information enzyme catalyses the racemization of D- and L-alanine Ovis aries ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Ovis aries