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Literature summary extracted from

  • Ogawa, H.; Fujioka, M.
    Purification and characterization of cytosolic and mitochondrial serine hydroxymethyltransferases from rat liver (1981), J. Biochem., 90, 381-390.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 D-alanine inactivates enzyme by converting the enzyme bound pyridoxal 5'-phosphate to pyridoxamine phosphate in a transamination reaction Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytoplasm
-
 Rattus norvegicus 5737
-
2.1.2.1 mitochondrion
-
 Rattus norvegicus 5739
-
2.1.2.1 additional information isozymes in cytosol and mitochondria Rattus norvegicus
-
-
2.1.2.1 additional information antigenically related Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 53700
-
 4 * 53700, mitochondria, SDS-PAGE Rattus norvegicus
2.1.2.1 56300
-
 4 * 56300, cytosol, SDS-PAGE Rattus norvegicus
2.1.2.1 200000 204000 mitochondria, gel filtration, PAGE, amino acid composition Rattus norvegicus
2.1.2.1 216000
-
 cytosol, PAGE Rattus norvegicus
2.1.2.1 230000
-
 cytosol, amino acid composition, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate Rattus norvegicus catalyzes interconversion of serine and glycine glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Rattus norvegicus
-
 Wistar strain
-
2.1.2.1 Rattus norvegicus Wistar
-
 Wistar strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 7.19
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Rattus norvegicus glycine + 5,10-methylenetetrahydrofolate + H2O
-
 r
2.1.2.1 L-serine + tetrahydrofolate catalyzes interconversion of serine and glycine Rattus norvegicus glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 additional information no: L-threonine Rattus norvegicus ?
-
 ?
2.1.2.1 additional information no: D-serine Rattus norvegicus ?
-
 ?
2.1.2.1 additional information enzyme catalyzes the pyridoxal 5'-phosphate dependent reversible cleavage of 3-hydroxy-alpha-amino acids Rattus norvegicus ?
-
 ?
2.1.2.1 additional information enzyme transaminates D-alanine to pyruvate and pyridoxamine phosphate Rattus norvegicus ?
-
 ?
2.1.2.1 additional information no: D-allothreonine Rattus norvegicus ?
-
 ?
2.1.2.1 additional information no: L-threonine Rattus norvegicus Wistar ?
-
 ?
2.1.2.1 additional information no: D-serine Rattus norvegicus Wistar ?
-
 ?
2.1.2.1 additional information enzyme catalyzes the pyridoxal 5'-phosphate dependent reversible cleavage of 3-hydroxy-alpha-amino acids Rattus norvegicus Wistar ?
-
 ?
2.1.2.1 additional information enzyme transaminates D-alanine to pyruvate and pyridoxamine phosphate Rattus norvegicus Wistar ?
-
 ?
2.1.2.1 additional information no: D-allothreonine Rattus norvegicus Wistar ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 homotetramer 4 * 56300, cytosol, SDS-PAGE Rattus norvegicus
2.1.2.1 homotetramer 4 * 53700, mitochondria, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 additional information
-
 pI, cytosolic enzyme: 4.95, pI, mitochondrial enzyme: 5.3 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate requirement, 4 mol per mol enzyme Rattus norvegicus
2.1.2.1 tetrahydrofolate
-
 Rattus norvegicus