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Literature summary extracted from

  • Eckermann, C.; Eichel, J.; Schroder, J.
    Plant methionine synthase: new insights into properties and expression (2000), Biol. Chem., 381, 695-703.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.14 0.08
-
 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
 Catharanthus roseus
2.1.1.14 0.35
-
 5-methyltetrahydropteroylmonoglutamate
-
 Catharanthus roseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.14 Mg2+ no stimulation Catharanthus roseus
2.1.1.14 phosphate
-
 Catharanthus roseus
2.1.1.14 Zn2+
-
 Catharanthus roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.14 85000
-
 gel filtration, recombinant enzyme Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.14 Catharanthus roseus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.14 cell culture
-
 Catharanthus roseus
-
2.1.1.14 cotyledon
-
 Catharanthus roseus
-
2.1.1.14 hypocotyl
-
 Catharanthus roseus
-
2.1.1.14 seedling low activity Catharanthus roseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
 Catharanthus roseus tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
 ?
2.1.1.14 5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Catharanthus roseus tetrahydropteroylmonoglutamate + L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.14 monomer 1 * 85000, SDS-PAGE, recombinant enzyme Catharanthus roseus