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Literature summary extracted from

  • Moloney, D.J.; Panin, V.M.; Johnston, S.H.; Chen, J.; Shao, U.; Wilson, R.; Wang, Y.; Stanley, P.; Irvine, K.D.; Haltwanger, R.S.; Vogt, T.F.
    Fringe is a glycosyltransferase that modifies Notch (2000), Nature, 406, 369-375.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.222 expressed in Drosophila S2 cells Drosophila melanogaster
2.4.1.222 Lunatic Fringe and Manic Fringe expressed in Chinese hamster ovary Lec1 cells Mammalia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.222 Manganese
-
 Drosophila melanogaster
2.4.1.222 Manganese
-
 Mammalia

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.222 Drosophila melanogaster
-
-
-
2.4.1.222 Mammalia
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.222 UDP-N-acetyl-alpha-D-glucosamine + [protein with EGF-like domain]-3-O-(alpha-L-fucosyl)-(L-serine/L-threonine) = UDP + [protein with EGF-like domain]-3-O-[N-acetyl-beta-D-glucosaminyl-(1->3)-alpha-L-fucosyl]-(L-serine/L-threonine) GlcNAc-transfer activity of Fringe is essential for it to modulate Notch signalling Drosophila melanogaster
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.222 additional information
-
 Lunic Fringe has a higher specific activity than either Drosophila Fringe and Manic Fringe Drosophila melanogaster
2.4.1.222 additional information
-
 Lunic Fringe has a higher specific activity than either Drosophila Fringe and Manic Fringe Mammalia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.222 epidermal growth factor-like sequence repeats-O-fucose + UDP-GlcNAc Drosophila Fringe, Lunic Fringe, Manic Fringe Drosophila melanogaster ?
-
 ?
2.4.1.222 epidermal growth factor-like sequence repeats-O-fucose + UDP-GlcNAc Drosophila Fringe, Lunic Fringe, Manic Fringe Mammalia ?
-
 ?
2.4.1.222 additional information further substrate: recombinant epidermal growth factor repeat from the serum protein factor VII modified with O-linked fucose Drosophila melanogaster ?
-
 ?
2.4.1.222 additional information further substrate: recombinant epidermal growth factor repeat from the serum protein factor VII modified with O-linked fucose Mammalia ?
-
 ?
2.4.1.222 additional information no substrates: p-nitrophenyl-galactose, p-nitrophenyl-glucose Drosophila melanogaster ?
-
 ?
2.4.1.222 additional information no substrates: p-nitrophenyl-galactose, p-nitrophenyl-glucose Mammalia ?
-
 ?
2.4.1.222 O-linked fucose on the epidermal growth factor-like sequence repeats of Notch + GlcNAc
-
 Drosophila melanogaster GlcNAc-beta-1,3-fucitol-Notch
-
 ?
2.4.1.222 O-linked fucose on the epidermal growth factor-like sequence repeats of Notch + GlcNAc endogenous Notch1, Manic Fringe Mammalia GlcNAc-beta-1,3-fucitol-Notch
-
 ?
2.4.1.222 p-nitrophenyl-alpha-L-fucose + UDP-GlcNAc p-nitrophenyl-alpha-L-fucose structurally mimics O-linked fucose, Lunic Fringe, Manic Fringe, Drosophila Fringe Drosophila melanogaster GlcNAc-beta-1,3-fucitol + p-nitrophenol + UDP
-
 ?
2.4.1.222 p-nitrophenyl-alpha-L-fucose + UDP-GlcNAc p-nitrophenyl-alpha-L-fucose structurally mimics O-linked fucose, Lunic Fringe, Manic Fringe, Drosophila Fringe Mammalia GlcNAc-beta-1,3-fucitol + p-nitrophenol + UDP
-
 ?
2.4.1.222 UDP-beta-D-GlcNAc + fucosyl-protein
-
 Drosophila melanogaster UDP + O-beta-D-GlcNAc-fucosyl-protein
-
 ?
2.4.1.222 UDP-beta-D-GlcNAc + fucosyl-protein
-
 Mammalia UDP + O-beta-D-GlcNAc-fucosyl-protein
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.222 37
-
 enzyme assay Drosophila melanogaster
2.4.1.222 37
-
 enzyme assay Mammalia