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Literature summary extracted from

  • Skiba, W.E.; Taylor, M.P.; Wells, M.S.; Mangum, J.H.; Awad, W.M.
    Human hepatic methionine biosynthesis. Purification and characterization of betaine:homocysteine S-methyltransferase (1982), J. Biol. Chem., 257, 14944-14948.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.5 divalent cations and 2-mercaptoethanol do not stabilize during purification Homo sapiens
2.1.1.5 substrates stabilize during purification Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.5 3,3-Dimethylbutyrate competitive to betaine Homo sapiens
2.1.1.5 Isovalerate competitive to betaine Homo sapiens
2.1.1.5 additional information not inhibitory: methionine or ethionine; product inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.5 0.1
-
 betaine
-
 Homo sapiens
2.1.1.5 0.12
-
 L-homocysteine
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.5 cytoplasm
-
 Homo sapiens 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.5 additional information
-
 amino acid composition Homo sapiens
2.1.1.5 45000
-
 6 * 45000, SDS-PAGE Homo sapiens
2.1.1.5 270000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.5 two forms of the enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.5 liver
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.5 -20°C, many months in 50% v/v glycerol Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.5 N,N,N-trimethylglycine + L-homocysteine
-
 Homo sapiens N,N-dimethylglycine + L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.5 hexamer 6 * 45000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.5 additional information
-
 significant heat stability Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.5 5.5
-
 above, 4 h stable at 37°C Homo sapiens