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Literature summary extracted from

  • Skiba, W.E.; Wells, M.S.; Mangum, J.H.; Awad, W.M.
    Betaine-homocysteine S-methyltransferase (human) (1987), Methods Enzymol., 143, 384-388.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.5 divalent cations and 2-mercaptoethanol do not stabilize during purification Homo sapiens
2.1.1.5 lyophilization inactivates Homo sapiens
2.1.1.5 substrates stabilize during purification Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.5 3,3-Dimethylbutyrate competitive to betaine Homo sapiens
2.1.1.5 Butyrate moderate, competitive to betaine Homo sapiens
2.1.1.5 Isovalerate competitive to betaine Homo sapiens
2.1.1.5 additional information no inhibition by sarcosine Homo sapiens
2.1.1.5 S-(delta-Carboxybutyl)-DL-homocysteine
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.5 45000
-
 6 * 45000, SDS-PAGE Homo sapiens
2.1.1.5 270000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.5 Equus caballus
-
-
-
2.1.1.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.5 two forms of the enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.5 kidney
-
 Homo sapiens
-
2.1.1.5 kidney
-
 Equus caballus
-
2.1.1.5 liver
-
 Homo sapiens
-
2.1.1.5 liver
-
 Equus caballus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.5 0.314
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.1.1.5 -20°C, over a year in 50% v/v glycerol Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.5 N,N,N-trimethylglycine + L-homocysteine
-
 Homo sapiens N,N-dimethylglycine + L-methionine
-
 ?
2.1.1.5 N,N,N-trimethylglycine + L-homocysteine
-
 Equus caballus N,N-dimethylglycine + L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.5 hexamer 6 * 45000, SDS-PAGE Homo sapiens