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Literature summary extracted from

  • Feng, T.Y.; Tu, J.; Kuo, T.T.
    Characterization of deoxycytidylate methyltransferase in Xanthomonas oryzae infected with bacteriophage Xp12 (1978), Eur. J. Biochem., 87, 29-36.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.54 Cu2+ above 1 mM Xanthomonas oryzae
2.1.1.54 Mg2+ above 50 mM Xanthomonas oryzae
2.1.1.54 Zn2+ above 1 mM Xanthomonas oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.54 0.0313
-
dCMP
-
Xanthomonas oryzae
2.1.1.54 0.0714
-
5,10-methylenetetrahydrofolate
-
Xanthomonas oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.54 Ca2+ 25 mM, slight stimulation Xanthomonas oryzae
2.1.1.54 Cu2+ below 1 mM, stimulation Xanthomonas oryzae
2.1.1.54 K+ 150 mM, stimulation Xanthomonas oryzae
2.1.1.54 Mg2+ stimulates below 25 mM Xanthomonas oryzae
2.1.1.54 Mn2+ has a similar effect in stimulation but not as strong as Mg2+ Xanthomonas oryzae
2.1.1.54 additional information among the buffers tested, 6-morpholinoethane sulfonate/Mg2+ is the best Xanthomonas oryzae
2.1.1.54 Zn2+ below 1 mM, stimulation Xanthomonas oryzae

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.54 Xanthomonas oryzae
-
phage Xp12-infected
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.54 phage Xp12-infected, partial Xanthomonas oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.54 0.11
-
-
Xanthomonas oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.54 5,10-methylenetetrahydrofolate + CMP
-
Xanthomonas oryzae dihydrofolate + 5-methylcytidylate
-
?
2.1.1.54 5,10-methylenetetrahydrofolate + CTP low activity Xanthomonas oryzae dihydrofolate + 5-methylcytidine 5'-triphosphate
-
?
2.1.1.54 5,10-methylenetetrahydrofolate + dCMP
-
Xanthomonas oryzae dihydrofolate + deoxy-5-methylcytidylate
-
?
2.1.1.54 5,10-methylenetetrahydrofolate + dCTP low activity Xanthomonas oryzae dihydrofolate + deoxy-5-methylcytidine 5'-triphosphate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.54 30
-
assay at Xanthomonas oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.54 50
-
3 min, 90% loss of activity Xanthomonas oryzae
2.1.1.54 70
-
3 min, complete loss of activity Xanthomonas oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.54 6
-
-
Xanthomonas oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.54 additional information
-
-
Xanthomonas oryzae