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Literature summary extracted from

  • Taylor, R.T.; Weissbach, H.
    N5-Methytetrahydrofolate-homocysteine (vitamin B12) methyltransferase (Escherichia coli B) (1971), Methods Enzymol., 17B, 379-388.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.13 0.0016
-
 S-adenosyl-L-methionine
-
 Escherichia coli
2.1.1.13 0.016
-
 L-homocysteine
-
 Escherichia coli
2.1.1.13 0.06
-
 5-methyltetrahydrofolate
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.13 140000
-
 sucrose density gradient centrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.13 Escherichia coli
-
 B
-
2.1.1.13 Escherichia coli B / ATCC 11303
-
 B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.13 B Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.13 additional information
-
 assay method Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.1.1.13 -20°C, 15-20 mg/ml protein, stable for at least 1 month Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine
-
 Escherichia coli tetrahydrofolate + L-methionine
-
 ?
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine
-
 Escherichia coli B / ATCC 11303 tetrahydrofolate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydrofolate + L-homocysteine
-
 Escherichia coli tetrahydrofolate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydrofolate + L-homocysteine
-
 Escherichia coli B / ATCC 11303 tetrahydrofolate + L-methionine
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Escherichia coli