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Literature summary extracted from

  • Nissen, P.; Martin-Nieto, J.
    Multimodal kinetics: Cyanobacterial nitrate reductase and other enzyme, transport and binding systems (1998), Physiol. Plant., 104, 503-511.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.7.2 strain 7120: gene encoding nitrate reductase is cloned and its complete amino acid sequence deduced Anabaena sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.2 additional information
-
 additional information
-
 Trichormus variabilis
1.7.7.2 additional information
-
 additional information
-
 Anabaena sp.
1.7.7.2 additional information
-
 additional information
-
 Synechocystis sp.
1.7.7.2 additional information
-
 additional information
-
 Nostoc sp.
1.7.7.2 additional information
-
 additional information
-
 Calothrix sp.
1.7.7.2 additional information
-
 additional information
-
 Fischerella muscicola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.7.2 nitrate + reduced ferredoxin Anabaena sp. first step in assimilatory reduction of nitrate nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin Nostoc sp. first step in assimilatory reduction of nitrate nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin Cyanobacteria first step in assimilatory reduction of nitrate nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin Anabaena sp. 7119 first step in assimilatory reduction of nitrate nitrite + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.2 Anabaena sp.
-
-
-
1.7.7.2 Anabaena sp.
-
 strains 7120 and M131
-
1.7.7.2 Anabaena sp. 7119
-
-
-
1.7.7.2 Calothrix sp.
-
 strains 7101 and 7601
-
1.7.7.2 Cyanobacteria
-
 heterocystous nitrogen-fixing cyanobacterial strains
-
1.7.7.2 Fischerella muscicola
-
-
-
1.7.7.2 Fischerella muscicola 1829
-
-
-
1.7.7.2 Nostoc sp.
-
 strains 29105 and 29150
-
1.7.7.2 Synechocystis sp.
-
 unicellular cyanobacterium
-
1.7.7.2 Trichormus variabilis
-
 strain ATCC 29413
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.2 185fold partial purification Trichormus variabilis
1.7.7.2 partial purification Synechocystis sp.
1.7.7.2 purified from a few cyanobacterial strains Cyanobacteria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.2 nitrate + reduced ferredoxin
-
 Trichormus variabilis nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin
-
 Anabaena sp. nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin
-
 Synechocystis sp. nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin
-
 Nostoc sp. nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin
-
 Calothrix sp. nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin
-
 Fischerella muscicola nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin depends on reduced ferredoxin for its activity, multimodal kinetic model: a single catalytic site functions in multiple kinetic modes Cyanobacteria nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin
-
 Anabaena sp. 7119 nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin
-
 Fischerella muscicola 1829 nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin first step in assimilatory reduction of nitrate Anabaena sp. nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin first step in assimilatory reduction of nitrate Nostoc sp. nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin first step in assimilatory reduction of nitrate Cyanobacteria nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin first step in assimilatory reduction of nitrate Anabaena sp. 7119 nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced methyl viologen
-
 Trichormus variabilis nitrite + oxidized methyl viologen
-
 ?
1.7.7.2 nitrate + reduced methyl viologen
-
 Synechocystis sp. nitrite + oxidized methyl viologen
-
 ?
1.7.7.2 nitrate + reduced methyl viologen
-
 Calothrix sp. nitrite + oxidized methyl viologen
-
 ?
1.7.7.2 nitrate + reduced methyl viologen
-
 Fischerella muscicola nitrite + oxidized methyl viologen
-
 ?
1.7.7.2 nitrate + reduced methyl viologen enzyme uses also reduced methyl viologen as reductant Anabaena sp. nitrite + oxidized methyl viologen
-
 ?
1.7.7.2 nitrate + reduced methyl viologen enzyme uses also reduced methyl viologen as reductant Nostoc sp. nitrite + oxidized methyl viologen
-
 ?
1.7.7.2 nitrate + reduced methyl viologen enzyme uses also reduced methyl viologen as reductant Cyanobacteria nitrite + oxidized methyl viologen
-
 ?
1.7.7.2 nitrate + reduced methyl viologen enzyme uses also reduced methyl viologen as reductant Anabaena sp. 7119 nitrite + oxidized methyl viologen
-
 ?
1.7.7.2 nitrate + reduced methyl viologen
-
 Fischerella muscicola 1829 nitrite + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.7.2 monomer
-
 Cyanobacteria

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.7.2 molybdenum cofactor
-
 Cyanobacteria