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Literature summary extracted from

  • Yamamoto, K.; Yoshitama, K.; Teramoto, S.
    Purification and characterization of polyphenol oxidase from callus cultures of Portulaca grandiflora (2002), Plant Biotechnol., 19, 95-101.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 2-hydroxy-2,4,6-cycloheptatrien-1-one 1 mM, complete inhibition of isoenzymes Ia, Ib and II Portulaca grandiflora
1.10.3.1 4-chloromercuribenzoate 1 mM, 96, 94 and 95% inhibition of isoenzymes Ia, Ib and II respectively Portulaca grandiflora
1.10.3.1 ascorbic acid
-
Portulaca grandiflora
1.10.3.1 Fe2+ 88%, 68% and 80% inhibition of isoenzymes Ia, Ib, and II, respectively Portulaca grandiflora
1.10.3.1 glutathione 1 mM, 98%, 95% and 96% inhibition of isoenzymes Ia, Ib, and II, respectively Portulaca grandiflora
1.10.3.1 iodoacetate 1 mM; 1 mM: 46%, 39% and 31% inhibition of isoenzymes Ia, Ib, and II, respectively Portulaca grandiflora
1.10.3.1 isoascorbic acid 1 mM, complete inhibition of isoenzymes Ia, Ib and II Portulaca grandiflora
1.10.3.1 kojic acid 1 mM complete inhibition of isoenzymes Ia, Ib and II Portulaca grandiflora
1.10.3.1 mimosine 1 mM, 88%, 79% and 82% inhibition of isoenzymes Ia, Ib, and II, respectively Portulaca grandiflora
1.10.3.1 additional information not inhibited by 2,2'-dipyridyl, 1,10-phenanthroline and EDTA Portulaca grandiflora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 2
-
L-Dopa isoenzyme Ia Portulaca grandiflora
1.10.3.1 2.2
-
L-Dopa isoenzyme Ib Portulaca grandiflora
1.10.3.1 3.5
-
L-Dopa isoenzyme II Portulaca grandiflora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 25000
-
1 * 27000 + 1 * 25000, isoenzyme II, SDS-PAGE Portulaca grandiflora
1.10.3.1 27000
-
1 * 27000 + 1 * 25000, isoenzyme II, SDS-PAGE Portulaca grandiflora
1.10.3.1 53000
-
isoenzyme II, gel filtration Portulaca grandiflora
1.10.3.1 65000
-
isoenzymes Ia and Ib, gel filtration Portulaca grandiflora
1.10.3.1 66000
-
1 * 66000, isoenzyme Ia, SDS-PAGE Portulaca grandiflora

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Portulaca grandiflora
-
isoenzymes Ia, Ib and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 isoenzymes Ia, Ib and II ammonium sulfate, DEAE-cellulose, Sephacryl S-200, hydroxyapatite, Mono Q Portulaca grandiflora

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 callus cell culture Portulaca grandiflora
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 additional information
-
isoenzyme Ia, 123 units/mg, isoenzyme Ib, 113 units/mg, isoenzyme II, 242 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.1 per min Portulaca grandiflora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 catechol + 1/2 O2 isoenzymes Ia, Ib and II, 90, 43 and 42% of activity with L-dopa respectively, no activity with L-tyrosine, D-tyrosine, hydroquinone and methylhydroquinone Portulaca grandiflora 1,2-benzoquinone + H2O
-
?
1.10.3.1 D-dopa + 1/2 O2 isoenzymes Ia, Ib and II, 54, 60 and 48% of activity with L-dopa respectively Portulaca grandiflora D-dopaquinone + H2O
-
?
1.10.3.1 L-dopa + 1/2 O2
-
Portulaca grandiflora L-dopaquinone + H2O
-
?
1.10.3.1 pyrogallol + O2 isoenzymes Ia, Ib and II, 5, 8 and 15% of activity with L-dopa respectively Portulaca grandiflora ?
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.1 dimer 1 * 27000 + 1 * 25000, isoenzyme II, SDS-PAGE Portulaca grandiflora
1.10.3.1 monomer 1 * 65000, isoenzyme Ib, SDS-PAGE Portulaca grandiflora
1.10.3.1 monomer 1 * 66000, isoenzyme Ia, SDS-PAGE Portulaca grandiflora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 5
-
isoenzymes Ia and Ib Portulaca grandiflora
1.10.3.1 6
-
isoenzyme II Portulaca grandiflora