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Literature summary extracted from

  • Shi, C.; Dai, Y.; Xu, X.; Xie, Y.; Liu, Q.
    The purification of polyphenol oxidase from tobacco (2002), Protein Expr. Purif., 24, 51-55.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 1.2
-
 catechol
-
 Nicotiana tabacum
1.10.3.1 1.2
-
 catechol pH 6.5 Nicotiana tabacum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 36500
-
 1 * 36500, MALDI-TOF mass spectrometry Nicotiana tabacum
1.10.3.1 42000
-
 1 * 42000, SDS-PAGE Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.1 additional information Nicotiana tabacum PPO II inhibits cultures of Escherichia coli and it accumulates on the wounded sites of tobacco leaves indicating that it may act as a defense role in plat defense system ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Nicotiana tabacum
-
-
-
1.10.3.1 Nicotiana tabacum
-
 polyphenol oxidase II, enzyme might act as an important defense system against the spread of pathogens at wounded sites
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1
-
 Nicotiana tabacum
1.10.3.1 ammonium sulfate, DEAE-Sephadex A-50, Sephadex G-75 Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 leaf
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 additional information
-
-
 Nicotiana tabacum
1.10.3.1 additional information
-
 4500 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.01 per min Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 catechol + 1/2 O2
-
 Nicotiana tabacum 1,2-benzoquinone + H2O
-
 ?
1.10.3.1 catechol + 1/2 O2 no activity with tyrosine, o-methoxyphenol, p-catechol and m-catechol Nicotiana tabacum 1,2-benzoquinone + H2O
-
 ?
1.10.3.1 chlorogenic acid + O2
-
 Nicotiana tabacum ?
-
 ?
1.10.3.1 chlorogenic acid + O2 50% of activity with catechol Nicotiana tabacum ?
-
 ?
1.10.3.1 additional information PPO II inhibits cultures of Escherichia coli and it accumulates on the wounded sites of tobacco leaves indicating that it may act as a defense role in plat defense system Nicotiana tabacum ?
-
 ?
1.10.3.1 additional information no activity with tyrosine, o-methoxyphenol, p-diphenol and m-diphenol Nicotiana tabacum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.1 monomer 1 * 42000, SDS-PAGE Nicotiana tabacum
1.10.3.1 monomer 1 * 36500, MALDI-TOF mass spectrometry Nicotiana tabacum

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 PPO II
-
 Nicotiana tabacum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 40
-
-
 Nicotiana tabacum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.1 20 65 94% of maximal activity at 30°C, 78% of maximal activity at 20°C, rapid decline of activity above 65°C Nicotiana tabacum
1.10.3.1 20 65 enzyme retains more than 50% of its activity from 20°C to 65°C Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 6
-
-
 Nicotiana tabacum
1.10.3.1 6
-
 rapid decrease in activity at higher and lower pH Nicotiana tabacum