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Literature summary extracted from

  • Wong, T.C.; Luh, B.S.; Whitaker, J.R.
    Isolation and characterization of polyphenol oxidase isozymes of clingstone peach (1971), Plant Physiol., 48, 19-23.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 ascorbic acid 0.066 mM, 26%, 59% and 96% inhibition of isoenzymes B, C, and D, respectively Prunus persica
1.10.3.1 CN- 0.165 mM, 84%, 70%, 100% and 40% inhibition of isoenzymes A, B, C and D, respectively Prunus persica
1.10.3.1 diethyldithiocarbamate 0.066 mM, 31%, 40%, 36% and 84% inhibition of isoenzymes A, B, C, and D, respectively Prunus persica
1.10.3.1 EDTA 100 mM, 33%, 60%, 81% and 35% inhibition of isoenzymes A, B, C and D, respectively Prunus persica
1.10.3.1 glutathione 0.066 mM, 2%, 22% and 84% inhibition of isoenzymes B, C, and D, respectively Prunus persica
1.10.3.1 NaCl 800 mM, 48%, 47%, 55% and 93% inhibition of isoenzymes A, B, C, and D, respectively Prunus persica
1.10.3.1 NaHSO3 0.066 mM, 64%, 50% and 27% inhibition of isoenzymes B, C, and D, respectively Prunus persica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 4.2
-
 catechol isoenzyme B Prunus persica
1.10.3.1 6.6
-
 catechol isoenzyme A Prunus persica
1.10.3.1 7
-
 catechol isoenzyme C Prunus persica
1.10.3.1 36
-
 catechol isoenzyme D Prunus persica

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Prunus persica
-
 peach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 isoenzymes A, B, C and D, acetone precipitate, DEAE-cellulose Prunus persica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 fruit
-
 Prunus persica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 additional information
-
 isoenzyme A, 0.0016 units, isoenzyme B, 0.011 units, isoenzyme C, 0.0013 units/mg, isoenzyme D, 0.0028 units, 1 unit is defined as absorbance change at 420 nm of 0.001 per min Prunus persica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 3,4,5-trihydroxybenzoic acid + O2 isoenzymes A-C Prunus persica ?
-
 ?
1.10.3.1 3,4,5-trihydroxybenzoic acid + O2 trivial name gallic acid Prunus persica ?
-
 ?
1.10.3.1 4-methylcatechol + O2 isoenzymes A-D Prunus persica 4-methyl-o-benzoquinone
-
 ?
1.10.3.1 caffeic acid + 1/2 O2 isoenzymes C and D Prunus persica caffeoyl quinone + H2O
-
 ?
1.10.3.1 catechol + 1/2 O2 isoenzymes A, B, C and D show no phenolase activity Prunus persica 1,2-benzoquinone + H2O
-
 ?
1.10.3.1 chlorogenic acid + O2 isoenzymes A-D Prunus persica ?
-
 ?
1.10.3.1 D-catechin + O2 isoenzymes A-D Prunus persica ?
-
 ?
1.10.3.1 dopamine + O2 isoenzymes A-D Prunus persica 4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.1 L-dopa + 1/2 O2 isoenzymes A-D Prunus persica L-dopaquinone + H2O
-
 ?
1.10.3.1 protocatechuic acid + O2 isoenzyme C Prunus persica ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.1 55
-
 half-lives of isoenzymes A, B and D: 5.4, 14,6 and 14.1 min respectively, isoenzyme C: stable for 50 min at 55°C, half-life at 76°C: 2.2 min Prunus persica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 6.5
-
 isoenzyme B Prunus persica
1.10.3.1 6.8
-
 isoenzyme A Prunus persica
1.10.3.1 7
-
 isoenzyme D Prunus persica
1.10.3.1 7.2
-
 isoenzyme C Prunus persica