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Literature summary extracted from

  • Massey, V.; Ghisla, S.; Kieschke, K.
    Studies on the reaction mechanism of lactate oxidase. Formation of two covalent flavin-substrate adducts on reaction with glycollate (1980), J. Biol. Chem., 255, 2796-2806.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Mycolicibacterium smegmatis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 glycolate + O2 the catalytic adduct is formed by hydrogen abstraction from the re-face of glycolate Mycolicibacterium smegmatis glyoxylate + H2O2
-
 ?
1.1.3.15 glyoxalate + O2
-
 Mycolicibacterium smegmatis oxalate + H2O2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.15 0.33
-
 glycolate pH 7.0 Mycolicibacterium smegmatis
1.1.3.15 1.1
-
 O2 pH 7.0 Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.15 7
-
 assay at Mycolicibacterium smegmatis