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Literature summary extracted from

  • Sun, W.; Williams, C.H., Jr.; Massey, V.
    Site-directed mutagenesis of glycine 99 to alanine in L-lactate monooxygenase from Mycobacterium smegmatis (1996), J. Biol. Chem., 271, 17226-17233.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.4 expressed in Escherichia coli Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.4 G99A (S)-lactate binds tighter to the G99A-mutant than to wild-type Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.4 (R)-lactate competitive inhibitor Mycolicibacterium smegmatis
1.13.12.4 2-Hydroxy-3-butynoate suicide substrate Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.4 0.014
-
 (S)-lactate calculated constant, G99A mutant Mycolicibacterium smegmatis
1.13.12.4 0.02
-
 (S)-lactate observed constant, G99A mutant Mycolicibacterium smegmatis
1.13.12.4 0.055
-
 O2 calculated constant, wild-type Mycolicibacterium smegmatis
1.13.12.4 0.071
-
 O2 observed constant, wild-type Mycolicibacterium smegmatis
1.13.12.4 0.83
-
 O2 observed constant, G99A mutant Mycolicibacterium smegmatis
1.13.12.4 0.92
-
 O2 calculated constant, G99A mutant Mycolicibacterium smegmatis
1.13.12.4 22
-
 (S)-lactate wild-type Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.4 43000
-
 8 * 43000, amino acid analysis Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.4 (S)-lactate + O2 Mycolicibacterium smegmatis
-
 acetate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.4 Mycolicibacterium smegmatis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.4 (S)-lactate + O2
-
 Mycolicibacterium smegmatis acetate + CO2 + H2O
-
 ?
1.13.12.4 (S)-lactate + O2 G99A-mutant, no decarboxylation Mycolicibacterium smegmatis pyruvate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.4 octamer 8 * 43000, amino acid analysis Mycolicibacterium smegmatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.12.4 0.0148
-
 (S)-lactate
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.4 FMN one FMN per subunit Mycolicibacterium smegmatis