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Literature summary extracted from

  • Xu, P.; Yano, T.; Yamamoto, K.; Suzuki, H.; Kumagai, H.
    Characterization of a lactate oxidase from a strain of gram negative bacterium from soil (1996), Appl. Biochem. Biotechnol., 56, 277-288.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.2 EDTA 40% activation at 1 mM Gram-negative soil bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.2 (R)-lactate at concentrations higher than 10 mM Gram-negative soil bacterium
1.1.3.2 FeSO4 86% inhibition at 1 mM Gram-negative soil bacterium
1.1.3.2 HgCl2 28% inhibition at 1 mM Gram-negative soil bacterium
1.1.3.2 iodoacetate 33% inhibition at 1 mM Gram-negative soil bacterium
1.1.3.2 L-leucine 29% inhibition at 1 mM Gram-negative soil bacterium
1.1.3.2 L-phenylalanine 28% inhibition at 1 mM Gram-negative soil bacterium
1.1.3.2 Lactate at concentrations higher than 20 mM Gram-negative soil bacterium
1.1.3.2 MnSO4 47% inhibition at 1 mM Gram-negative soil bacterium
1.1.3.2 p-chloromercuribenzoate 21% inhibition at 1 mM Gram-negative soil bacterium
1.1.3.2 ZnSO4 28% inhibition at 1 mM Gram-negative soil bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.2 0.14
-
 (R)-lactate
-
 Gram-negative soil bacterium
1.1.3.2 0.2
-
 Lactate
-
 Gram-negative soil bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.2 45000
-
 ? * 45000, SDS-PAGE Gram-negative soil bacterium
1.1.3.2 204000
-
 gel filtration Gram-negative soil bacterium

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.2 Gram-negative soil bacterium
-
-
-
1.1.3.2 Gram-negative soil bacterium KY6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.2
-
 Gram-negative soil bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.2 (R)-lactate + 2,6-dichlorophenolindophenol no decarboxylation Gram-negative soil bacterium ?
-
 ?
1.1.3.2 (R)-lactate + 2,6-dichlorophenolindophenol no decarboxylation Gram-negative soil bacterium KY6 ?
-
 ?
1.1.3.2 (S)-lactate + 2,6-dichlorophenolindophenol no decarboxylation Gram-negative soil bacterium pyruvate + ?
-
 ?
1.1.3.2 (S)-lactate + 2,6-dichlorophenolindophenol no decarboxylation Gram-negative soil bacterium KY6 pyruvate + ?
-
 ?
1.1.3.2 glycolate + 2,6-dichlorophenolindophenol no decarboxylation Gram-negative soil bacterium ?
-
 ?
1.1.3.2 glycolate + 2,6-dichlorophenolindophenol no decarboxylation Gram-negative soil bacterium KY6 ?
-
 ?
1.1.3.2 L-2-hydroxyisocaproate + 2,6-dichlorophenolindophenol no decarboxylation Gram-negative soil bacterium ?
-
 ?
1.1.3.2 L-2-hydroxyisocaproate + 2,6-dichlorophenolindophenol no decarboxylation Gram-negative soil bacterium KY6 ?
-
 ?
1.1.3.2 L-malic acid + 2,6-dichlorophenolindophenol no decarboxylation Gram-negative soil bacterium ?
-
 ?
1.1.3.2 lactate + 2,6-dichlorophenolindophenol no decarboxylation Gram-negative soil bacterium ?
-
 ?
1.1.3.2 lactate + 2,6-dichlorophenolindophenol no decarboxylation Gram-negative soil bacterium KY6 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.2 ? ? * 45000, SDS-PAGE Gram-negative soil bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.2 70
-
-
 Gram-negative soil bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.2
-
 45 stable for 10 min Gram-negative soil bacterium
1.1.3.2 55
-
 50% activity after 10 min Gram-negative soil bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.2 7.7
-
-
 Gram-negative soil bacterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.2 5.7 9.5 stable for 24 h at 4°C Gram-negative soil bacterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.2 FMN
-
 Gram-negative soil bacterium