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Literature summary extracted from

  • Jiang, H.; Parales, R.E.; Lynch, N.A.; Gibson, D.T.
    Site-directed mutagenesis of conserved amino acids in the alpha subunit of toluene dioxygenase: potential mononuclear non-heme iron coordination sites (1996), J. Bacteriol., 178, 3133-3139.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.11 D219A mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol Pseudomonas putida
1.14.12.11 E214A mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol Pseudomonas putida
1.14.12.11 H222A mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol Pseudomonas putida
1.14.12.11 H228A mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol Pseudomonas putida
1.14.12.11 Y221A mutation at alpha-subunit of oxygenase component, 42% of the activity of the wild-type enzyme, formation of cis-toluene dihydrodiol is reduced Pseudomonas putida
1.14.12.11 Y266A mutation at alpha-subunit of oxygenase component, 12% of the activity of the wild-type enzyme, formation of cis-toluene dihydrodiol is reduced Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.11 Iron enzyme requires mononuclear iron for activity Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida
-
 F1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.11 beta-subunit of oxygenase component Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 toluene + NADH + O2
-
 Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.11 NADH
-
 Pseudomonas putida