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Literature summary extracted from

  • Wackett, L.P.
    Toluene dioxygenase from Pseudomonas putida F1 (1990), Methods Enzymol., 188, 39-45.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.11 Iron the oxygenase component contains 4-6 iron atoms per holoenzyme, the ferredoxin component contains one [2Fe-s2S] cluster of the Rieske type Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.11 additional information
-
 three-component enzyme: 1. reductaseTOL, 2. ferredoxinTOL, 3. a complex iron-sulfur protein Pseudomonas putida
1.14.12.11 15300
-
 alpha2beta2, 2 * 46000 + 2 * 15300, oxygenase component of toluene dioxygenase Pseudomonas putida
1.14.12.11 15500
-
 1 * 15500, ferredoxinTOL component of the toluene dioxygenase, SDS-PAGE Pseudomonas putida
1.14.12.11 46000
-
 1 * 46000, NADH-ferredoxinTOL reductase component, SDS-PAGE Pseudomonas putida
1.14.12.11 46000
-
 alpha2beta2, 2 * 46000 + 2 * 15300, oxygenase component of toluene dioxygenase Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida
-
 F1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.11 ferredoxinTOL component of the toluene dioxygenase Pseudomonas putida
1.14.12.11 NADH-ferredoxinTOL reductase component of toluene dioxygenase Pseudomonas putida
1.14.12.11 oxygenase component, ISPTOL Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.11 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 toluene + NADH + O2
-
 Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.11 monomer 1 * 15500, ferredoxinTOL component of the toluene dioxygenase, SDS-PAGE Pseudomonas putida
1.14.12.11 monomer 1 * 46000, NADH-ferredoxinTOL reductase component, SDS-PAGE Pseudomonas putida
1.14.12.11 tetramer alpha2beta2, 2 * 46000 + 2 * 15300, oxygenase component of toluene dioxygenase Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.11 FAD NADH-ferredoxinTOL reductase component contains 1 mol of noncovalently bound FAD per mol of protein Pseudomonas putida
1.14.12.11 NADH
-
 Pseudomonas putida