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Literature summary extracted from

  • Stephens, G.M.; Sidebotham, J.M.; Mann, N.H.; Dalton, H.
    Cloning and expression in Escherichia coli of the toluene dioxygenase gene from Pseudomonas putida (1989), FEMS Microbiol. Lett., 57, 295-300.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.11 cloned and expressed in Escherichia coli HB101 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.11 toluene + NADH + O2 Pseudomonas putida initial enzyme of toluene catabolism (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
?
1.14.12.11 toluene + NADH + O2 Pseudomonas putida NCIB11767 initial enzyme of toluene catabolism (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida
-
NCIB11767
-
1.14.12.11 Pseudomonas putida NCIB11767
-
NCIB11767
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 toluene + NADH + O2
-
Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
?
1.14.12.11 toluene + NADH + O2 initial enzyme of toluene catabolism Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
?
1.14.12.11 toluene + NADH + O2
-
Pseudomonas putida NCIB11767 (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
?
1.14.12.11 toluene + NADH + O2 initial enzyme of toluene catabolism Pseudomonas putida NCIB11767 (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.11 NADH
-
Pseudomonas putida