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Literature summary extracted from

  • Wackett, L.P.; Kwart, L.D.; Gibson, D.T.
    Benzylic monooxygenation catalyzed by toluene dioxygenase from Pseudomonas putida (1988), Biochemistry, 27, 1360-1367.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida
-
-
-
1.14.12.12 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 indane + NADH + O2 monooxygenase reaction of toluene dioxygenase Pseudomonas putida (-)-(1R)-indanol + NAD+ 84% enantiomeric excess of (-)-(1R)-indanol, 70% of the oxygen in 1-indanol is derived from water ?
1.14.12.11 indene + O2 + NADH monooxygenase reaction of toluene dioxygenase Pseudomonas putida (-)-cis-(1S,2R)-dihydroxyindan + (+)-(1S)-indenol + ? in addition the enzyme catalyzes the dioxygen addition of the nonaromatic double bond of indene to form cis-1,2-indanediol. The oxygen atom in 1-indenol and cis,1,2-indanediol is derived from molecular oxygen ?
1.14.12.11 toluene + NADH + O2
-
Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
?
1.14.12.12 indan + NADH + H+ + O2
-
Pseudomonas putida 1-indanol + NAD+
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.11 NADH
-
Pseudomonas putida