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Literature summary extracted from
- Toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography (1979), Biochem. Biophys. Res. Commun., 91, 1131-1139.
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.11 | Fe2+ | required | Pseudomonas putida |  |
| 1.14.12.11 | Iron | iron-sulfur protein contains 2 gatom of iron and 2 gatom of acid-labile sulfur per mol of protein | Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.11 | additional information | - |
the enzyme system consists of monomeric reductaseTOL and ferredoxinTOL and a dimeric terminal oxygenase | Pseudomonas putida |
| 1.14.12.11 | 20800 | - |
x * 52500 + x * 20800, oxygenase component, SDS-PAGE | Pseudomonas putida |
| 1.14.12.11 | 52500 | - |
x * 52500 + x * 20800, oxygenase component, SDS-PAGE | Pseudomonas putida |
| 1.14.12.11 | 151000 | - |
oxygenase component, gel filtration | Pseudomonas putida |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.12.11 | Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.12.11 | oxygenase component, ISPTOL | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.11 | 0.0075 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.12.11 | toluene + NADH + O2 | - |
Pseudomonas putida | (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.12.11 | ? | x * 52500 + x * 20800, oxygenase component, SDS-PAGE | Pseudomonas putida |
| 1.14.12.11 | More | the enzyme system consists of monomeric reductaseTOL, a ferredoxinTOL and a dimeric terminal oxygenase | Pseudomonas putida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.12.11 | ISPTOL | oxygenase component of the toluene dioxygenase multienzyme system | Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.11 | NADH | - |
Pseudomonas putida | |
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Release 2023.1 (January 2023)
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