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Literature summary extracted from
- Purification, characterization, and kinetic analysis of a 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis from Catharanthus roseus (1993), J. Biol. Chem., 268, 5504-5511.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.20 | ascorbic acid | - |
Catharanthus roseus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.20 | CO | 7.5 mM, 50% inhibition | Catharanthus roseus | |
| 1.14.11.20 | desacetylvindoline | product inhibition, noncompetitive vs. 2-oxoglutarate | Catharanthus roseus | |
| 1.14.11.20 | succinate | product inhibition, competitive vs. 2-oxoglutarate, noncompetitive vs. desacetoxyvindoline | Catharanthus roseus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.20 | 0.000003 | - |
desacetoxyvindoline | - |
Catharanthus roseus | |
| 1.14.11.20 | 0.0085 | - |
Fe2+ | - |
Catharanthus roseus | |
| 1.14.11.20 | 0.045 | - |
O2 | - |
Catharanthus roseus | |
| 1.14.11.20 | 0.045 | - |
2-oxoglutarate | - |
Catharanthus roseus | |
| 1.14.11.20 | 0.2 | - |
ascorbate | - |
Catharanthus roseus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.20 | Fe2+ | - |
Catharanthus roseus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.20 | 44700 | - |
1 * 44700, SDS-PAGE | Catharanthus roseus |
| 1.14.11.20 | 45000 | - |
- |
Catharanthus roseus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.20 | Catharanthus roseus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.11.20 | Sephadex G-100, Green 19-agarose, hydroxylapatite, 2-oxoglutarate-Sepharose, Mono-Q | Catharanthus roseus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.20 | 0.00517 | - |
- |
Catharanthus roseus |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.11.20 | monomer | 1 * 44700, SDS-PAGE | Catharanthus roseus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.20 | 2-oxoglutarate | - |
Catharanthus roseus | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.20 | 0.115 | - |
deacetylvindoline | - |
Catharanthus roseus | |
| 1.14.11.20 | 9 | - |
succinate | - |
Catharanthus roseus | |
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