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Literature summary extracted from

  • Demady, D.R.; Jianmongkol, S.; Vuletich, J.L.; Bender, A.T.; Osawa, Y.
    Agmatine enhances the NADPH oxidase activity of neuronal NO synthase and leads to oxidative inactivation of the enzyme (2001), Mol. Pharmacol., 59, 24-29.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.13.39 pharmacology NO synthase can be used to gain insight into the biological role of endogenous agmatine Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 neuronal enzyme expressed in Spodoptera frugiperda cells via baculovirus infection Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 agmatine at lower concentration than the Ki value agmatine leads to time-, concentration-, NADPH- and calmodulin-dependent inhibition of the neuronal enzyme in presence of calmodulin; causes an increase in NADPH oxidase activity of the enzyme Homo sapiens
1.14.13.39 H2O2 alters heme group, decrease in activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.39 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Iron 0.88 mol per mol of recombinant enzyme monomer Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 wild-type recombinant from insect cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 neuron
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.39 additional information
-
 assay method Homo sapiens
1.14.13.39 1
-
 purified enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Homo sapiens 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.13.39 0.66
-
 agmatine at lower concentration than the Ki value agmatine leads to time-, concentration-, NADPH- and calmodulin-dependent inhibition of the neuronal enzyme in presence of calmodulin Homo sapiens