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Literature summary extracted from

  • Xia, Y.; Tsai, A.L.; Berka, V.; Zweier, J.L.
    Superoxide generation from endothelial nitric-oxide synthase. A Ca2+/calmodulin-dependent and tetrahydrobiopterin regulatory process (1998), J. Biol. Chem., 273, 25804-25808.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 wild-type endothelial enzyme is expressed in insect cells via baculovirus infection Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 cyanide heme-blocker inhibits superoxide formation after pretreatment of the enzyme Homo sapiens
1.14.13.39 imidazole heme-blocker inhibits superoxide formation after pretreatment of the enzyme Homo sapiens
1.14.13.39 Ngamma-nitro-L-arginine methyl ester only L-isomer, inhibits NO and citrulline production from L-arginine as well as superoxide formation in absence of tetrahydropterin Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+ Ca2+/calmodulin is required for citrulline/NO formation and for superoxide formation in absence of tetrahydropterin Homo sapiens
1.14.13.39 Iron
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.39 135000
-
 x * 135000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 wild-type recombinant from insect cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 endothelium
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Homo sapiens 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 additional information enzyme can also Ca2+/calmodulin-dependently produce superoxide in absence of tetrahydropterin and in depletion of L-arginine, which is inhibited by tetrahydropterin, cyanide and imidazole Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.39 ? x * 135000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 23
-
 about, assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.39 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 Calmodulin Ca2+/calmodulin is required for superoxide formation in absence of tetrahydropterin Homo sapiens
1.14.13.39 NADPH
-
 Homo sapiens