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Literature summary extracted from
- Nitrogenase complex and its components (1972), Methods Enzymol., 24B, 456-470.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.18.6.1 | nitrogenase complex is more stable than either the MoFe protein or the Fe protein alone | Azotobacter vinelandii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.18.6.1 | ADP | above 5 mM | Azotobacter vinelandii |  |
| 1.18.6.1 | ATP | - |
Azotobacter vinelandii | |
| 1.18.6.1 | Ca2+ | - |
Azotobacter vinelandii | |
| 1.18.6.1 | CO | - |
Azotobacter vinelandii | |
| 1.18.6.1 | H2 | - |
Azotobacter vinelandii | |
| 1.18.6.1 | hydrazine | and derivatives | Azotobacter vinelandii | |
| 1.18.6.1 | additional information | e.g. above 50 mM NaCl; high ionic strength inhibits | Azotobacter vinelandii | |
| 1.18.6.1 | phosphate | above 30 mM | Azotobacter vinelandii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.18.6.1 | 0.1 | - |
N2 | with C2H2 | Azotobacter vinelandii | |
| 1.18.6.1 | 0.3 | - |
C2H2 | - |
Azotobacter vinelandii | |
| 1.18.6.1 | 0.3 | - |
ATP | with C2H2 | Azotobacter vinelandii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.18.6.1 | Co2+ | can replace Mg2+, but is less effective | Azotobacter vinelandii | |
| 1.18.6.1 | Fe2+ | can replace Mg2+, but is less effective | Azotobacter vinelandii | |
| 1.18.6.1 | Iron | - |
Azotobacter vinelandii | |
| 1.18.6.1 | Mg2+ | Mg2+ required for MgATP complex | Azotobacter vinelandii | |
| 1.18.6.1 | Mn2+ | can replace Mg2+, but is less effective | Azotobacter vinelandii | |
| 1.18.6.1 | Molybdenum | - |
Azotobacter vinelandii | |
| 1.18.6.1 | Ni2+ | can replace Mg2+, but is less effective | Azotobacter vinelandii | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.18.6.1 | 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Azotobacter vinelandii | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.18.6.1 | Azotobacter vinelandii | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.18.6.1 | extreme O2 lability, susceptibility to O2 increases with purification, but is retarded in presence of MgCl2 | Azotobacter vinelandii |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.18.6.1 | - |
Azotobacter vinelandii |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.18.6.1 | 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Azotobacter vinelandii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.18.6.1 | additional information | - |
- |
Azotobacter vinelandii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.18.6.1 | 0°C, anaerobic conditions, FeMo protein stable | Azotobacter vinelandii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.18.6.1 | 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O | - |
Azotobacter vinelandii | 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate | - |
? | |
| 1.18.6.1 | 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
| 1.18.6.1 | reduced ferredoxin + H+ + ATP | in absence of other acceptors | Azotobacter vinelandii | oxidized ferredoxin + H2 + ADP + phosphate | - |
? | |
| 1.18.6.1 | reduced ferredoxin + H+ + CH3NC + ATP | - |
Azotobacter vinelandii | oxidized ferredoxin + CH4 + C2H4 + C3H6 + C3H8 + CH3NH2 + ADP + phosphate | - |
? | |
| 1.18.6.1 | reduced ferredoxin + H+ + N3- + ATP | - |
Azotobacter vinelandii | oxidized ferredoxin + NH3 + N2 + ADP + phosphate | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.18.6.1 | 30 | - |
assay at | Azotobacter vinelandii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.18.6.1 | additional information | - |
Fe protein: cold labile | Azotobacter vinelandii |
| 1.18.6.1 | 22 | - |
Fe protein, half-life: 18 h | Azotobacter vinelandii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.18.6.1 | 7.3 | - |
- |
Azotobacter vinelandii |
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