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Literature summary extracted from
- Isolation and characterization of nitrogenase from Klebsiella pneumoniae (1986), Methods Enzymol., 118, 511-519.
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.18.6.1 | Iron | - |
Klebsiella pneumoniae |  |
| 1.18.6.1 | Mg2+ | Mg2+ required for MgATP complex | Klebsiella pneumoniae | |
| 1.18.6.1 | Molybdenum | - |
Klebsiella pneumoniae | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.18.6.1 | 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Klebsiella pneumoniae | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.18.6.1 | Klebsiella pneumoniae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.18.6.1 | both components | Klebsiella pneumoniae |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.18.6.1 | 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Klebsiella pneumoniae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.18.6.1 | 2.082 | - |
purified component II | Klebsiella pneumoniae |
| 1.18.6.1 | 2.16 | - |
purified component I | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.18.6.1 | 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O | - |
Klebsiella pneumoniae | 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate | - |
? | |
| 1.18.6.1 | 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Klebsiella pneumoniae | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
| 1.18.6.1 | dithionite + H+ + N2 + ATP | in vitro substrate | Klebsiella pneumoniae | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.18.6.1 | 30 | - |
assay at | Klebsiella pneumoniae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.18.6.1 | 7.4 | - |
assay at | Klebsiella pneumoniae |
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