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Literature summary extracted from

  • Palmer, G.
    Iron-sulfur proteins (1975), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 12, 1-56.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.6.1 O2
-
 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Ca2+ 1.2 gatom per mol of MoFe protein Klebsiella pneumoniae
1.18.6.1 Cu2+ 1.4 gatom per mol of MoFe protein Klebsiella pneumoniae
1.18.6.1 Iron 4 atoms of iron per molecule of Fe protein Klebsiella pneumoniae
1.18.6.1 Iron 17.5 gatom per mol of MoFe protein Klebsiella pneumoniae
1.18.6.1 Mg2+ 1.8 gatom per mol of MoFe protein Klebsiella pneumoniae
1.18.6.1 Mg2+ Mg2+ required for MgATP complex Klebsiella pneumoniae
1.18.6.1 Molybdenum 1 gatom per mol of MoFe protein Klebsiella pneumoniae
1.18.6.1 Zn2+ 0.8 gatom per mol of MoFe protein Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.6.1 additional information
-
 enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Klebsiella pneumoniae
1.18.6.1 62000
-
 Fe protein, gel filtration Klebsiella pneumoniae
1.18.6.1 220000
-
 MoFe protein, gel filtration Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Klebsiella pneumoniae
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Klebsiella pneumoniae
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.18.6.1 extreme O2 lability, t1/2: 10 min, MoFe protein 45 s, Fe protein Klebsiella pneumoniae

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Klebsiella pneumoniae
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Klebsiella pneumoniae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.6.1 additional information
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Klebsiella pneumoniae 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?