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Literature summary extracted from

  • Vignais, P.M.; Colbeau, A.; Willison, J.C.; Jouanneau, Y.
    Hydrogenase, nitrogenase, and hydrogen metabolism in the photosynthetic bacteria (1985), Adv. Microb. Physiol., 26, 155-234.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.18.6.1 additional information Fe protein contains an adenine-like molecule, a pentose moiety and a phosphate residue covalently attached to the molecule Rhodobacter capsulatus
1.18.6.1 additional information Fe protein contains an adenine-like molecule, a pentose moiety and a phosphate residue covalently attached to the molecule Rhodospirillum rubrum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.6.1 glutamine
-
 Rhodobacter capsulatus
1.18.6.1 glutamine
-
 Rhodospirillum rubrum
1.18.6.1 NH4+ immediate inhibition, repression of induction Rhodobacter capsulatus
1.18.6.1 NH4+ immediate inhibition, repression of induction Rhodospirillum rubrum
1.18.6.1 O2
-
 Azotobacter vinelandii
1.18.6.1 O2
-
 Clostridium pasteurianum
1.18.6.1 O2
-
 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Iron
-
 Clostridium pasteurianum
1.18.6.1 Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Rhodobacter capsulatus
1.18.6.1 Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Klebsiella pneumoniae
1.18.6.1 Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Azotobacter vinelandii
1.18.6.1 Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Rhodospirillum rubrum
1.18.6.1 Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Allochromatium vinosum
1.18.6.1 Iron iron content of MoFe protein: 30 Azotobacter vinelandii
1.18.6.1 Iron the MoFe protein contains 2 molybdenum, about 30 iron and 30 inorganic sulphur atoms, 16 of the 30 Fe atoms are associated with S2- in four cubic [4Fe-4S] clusters, the remaining metal atoms are arranged in two copies of a cofactor called FeMo cofactor, FeMoCo, with a minimum stoichiometry of MoFe6S8-9 Azotobacter vinelandii
1.18.6.1 Iron 2.7-4.1 mol per mol of Fe protein Rhodobacter capsulatus
1.18.6.1 Iron 20 atoms of iron per molecule of MoFe protein Rhodospirillum rubrum
1.18.6.1 Iron 27.7 atoms of iron per molecule of MoFe protein Rhodobacter capsulatus
1.18.6.1 Molybdenum
-
 Clostridium pasteurianum
1.18.6.1 Molybdenum 1.2-1.3 gatom per mol of MoFe protein Rhodobacter capsulatus
1.18.6.1 Molybdenum 1.7 gatom per mol of MoFe protein Rhodospirillum rubrum
1.18.6.1 Molybdenum 2 gatom per mol of MoFe protein Azotobacter vinelandii
1.18.6.1 Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Rhodobacter capsulatus
1.18.6.1 Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Klebsiella pneumoniae
1.18.6.1 Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Azotobacter vinelandii
1.18.6.1 Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Rhodospirillum rubrum
1.18.6.1 Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Allochromatium vinosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.6.1 additional information
-
-
 Clostridium pasteurianum
1.18.6.1 additional information
-
 enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Rhodobacter capsulatus
1.18.6.1 additional information
-
 enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Klebsiella pneumoniae
1.18.6.1 additional information
-
 enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Azotobacter vinelandii
1.18.6.1 additional information
-
 enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Rhodospirillum rubrum
1.18.6.1 additional information
-
 enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Allochromatium vinosum
1.18.6.1 61500
-
 Fe protein Rhodospirillum rubrum
1.18.6.1 63000
-
 Fe protein Rhodobacter capsulatus
1.18.6.1 63000
-
 Fe protein Azotobacter vinelandii
1.18.6.1 230000
-
 MoFe protein Rhodobacter capsulatus
1.18.6.1 230000
-
 MoFe protein Azotobacter vinelandii
1.18.6.1 230000
-
 MoFe protein Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Rhodobacter capsulatus
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Klebsiella pneumoniae
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Clostridium pasteurianum
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Rhodospirillum rubrum
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Allochromatium vinosum
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Allochromatium vinosum
-
-
-
1.18.6.1 Azotobacter vinelandii
-
-
-
1.18.6.1 Clostridium pasteurianum
-
-
-
1.18.6.1 Klebsiella pneumoniae
-
-
-
1.18.6.1 Rhodobacter capsulatus
-
-
-
1.18.6.1 Rhodospirillum rubrum
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.18.6.1 extreme sensitivity to O2 Rhodobacter capsulatus
1.18.6.1 extreme sensitivity to O2 Klebsiella pneumoniae
1.18.6.1 extreme sensitivity to O2 Clostridium pasteurianum
1.18.6.1 extreme sensitivity to O2 Azotobacter vinelandii
1.18.6.1 extreme sensitivity to O2 Rhodospirillum rubrum
1.18.6.1 extreme sensitivity to O2 Allochromatium vinosum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Rhodobacter capsulatus
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Klebsiella pneumoniae
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Clostridium pasteurianum
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Azotobacter vinelandii
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Rhodospirillum rubrum
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Allochromatium vinosum
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Rhodobacter capsulatus
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Klebsiella pneumoniae
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Clostridium pasteurianum
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter vinelandii
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Rhodospirillum rubrum
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Allochromatium vinosum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Rhodobacter capsulatus 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Klebsiella pneumoniae 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Clostridium pasteurianum 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Azotobacter vinelandii 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Rhodospirillum rubrum 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Allochromatium vinosum 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
1.18.6.1 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
 Rhodobacter capsulatus 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
 ?
1.18.6.1 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
 Klebsiella pneumoniae 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
 ?
1.18.6.1 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
 Clostridium pasteurianum 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
 ?
1.18.6.1 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
 Azotobacter vinelandii 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
 ?
1.18.6.1 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
 Rhodospirillum rubrum 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
 ?
1.18.6.1 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
 Allochromatium vinosum 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Rhodobacter capsulatus 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Klebsiella pneumoniae 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Clostridium pasteurianum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Rhodospirillum rubrum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Allochromatium vinosum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.6.1 dimer
-
 Klebsiella pneumoniae
1.18.6.1 dimer
-
 Clostridium pasteurianum
1.18.6.1 dimer
-
 Azotobacter vinelandii
1.18.6.1 dimer
-
 Rhodospirillum rubrum
1.18.6.1 dimer
-
 Allochromatium vinosum
1.18.6.1 More
-
 Clostridium pasteurianum
1.18.6.1 More enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Rhodobacter capsulatus
1.18.6.1 More enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Klebsiella pneumoniae
1.18.6.1 More enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Azotobacter vinelandii
1.18.6.1 More enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Rhodospirillum rubrum
1.18.6.1 More enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Allochromatium vinosum
1.18.6.1 tetramer
-
 Clostridium pasteurianum
1.18.6.1 tetramer alpha: 55000, beta: 59500 Rhodobacter capsulatus
1.18.6.1 tetramer MoFe protein is an alpha2beta2-tetramer Rhodobacter capsulatus
1.18.6.1 tetramer MoFe protein is an alpha2beta2-tetramer Klebsiella pneumoniae
1.18.6.1 tetramer MoFe protein is an alpha2beta2-tetramer Azotobacter vinelandii
1.18.6.1 tetramer MoFe protein is an alpha2beta2-tetramer Rhodospirillum rubrum
1.18.6.1 tetramer MoFe protein is an alpha2beta2-tetramer Allochromatium vinosum
1.18.6.1 tetramer alpha: 58500, beta: 58500 Rhodospirillum rubrum