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Literature summary extracted from

  • Peters, R.J.; Croteau, R.B.
    Abietadiene synthase catalysis: conserved residues involved in protonation-initiated cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate (2002), Biochemistry, 41, 1836-1842.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.12 expression in Escherichia coli Abies grandis

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.18 D361A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 D402A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 D402E lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 D402N lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 D404A
-
 Abies grandis
4.2.3.18 D404E lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 D404N lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 D405A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 D405E lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 D405N lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 E499A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 R365A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 R411A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 R454A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 W358A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
4.2.3.18 Y520A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 D361A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 D402A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 D402E lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 D402N lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 D404A
-
 Abies grandis
5.5.1.12 D404E lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 D404N lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 D405A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 D405E lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 D405N lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 E499A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 R365A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 R411A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 R454A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 W358A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
5.5.1.12 Y520A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.18 14,15-dihydro-15-aza-geranylgeranyl diphosphate
-
 Abies grandis
4.2.3.18 geranylgeranyl diphosphate
-
 Abies grandis
5.5.1.12 14,15-dihydro-15-aza-geranylgeranyl diphosphate
-
 Abies grandis
5.5.1.12 geranylgeranyl diphosphate
-
 Abies grandis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.18 0.0002
-
 copalyl diphosphate mutants D404N, R411A Abies grandis
4.2.3.18 0.0003
-
 copalyl diphosphate mutants D402A, D402N, D404E Abies grandis
4.2.3.18 0.0004
-
 copalyl diphosphate mutants W358A, D361A, D404A, D405E Abies grandis
4.2.3.18 0.0005
-
 copalyl diphosphate mutants D402E, D405E, R454A, E499A Y520A Abies grandis
4.2.3.18 0.0007
-
 copalyl diphosphate mutants D405N, R365A Abies grandis
4.2.3.18 132
-
 copalyl diphosphate
-
 Abies grandis
5.5.1.12 0.0004
-
 geranylgeranyl diphosphate W358A Abies grandis
5.5.1.12 0.0006
-
 geranylgeranyl diphosphate D405N Abies grandis
5.5.1.12 0.0008
-
 geranylgeranyl diphosphate D361A Abies grandis
5.5.1.12 0.001
-
 geranylgeranyl diphosphate D402A, D402E, D405E, R454A Abies grandis
5.5.1.12 0.002
-
 geranylgeranyl diphosphate E499A, R411A, R365A Abies grandis
5.5.1.12 0.003
-
 geranylgeranyl diphosphate wild-type, D405A, Y520A Abies grandis
5.5.1.12 0.007
-
 geranylgeranyl diphosphate D404A Abies grandis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.18 Mg2+ 10 mM selectively enables the conversion of copalyl diphosphate Abies grandis
5.5.1.12 Mg2+ 10 mM selectively enabled the conversion of copalyl diphosphate Abies grandis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.18 (+)-copalyl-diphosphate Abies grandis
-
 (-)-abietadiene + diphosphate
-
 ?
5.5.1.12 geranylgeranyl diphosphate Abies grandis
-
 (+)-copalyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.18 Abies grandis
-
-
-
5.5.1.12 Abies grandis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.18 (+)-copalyl-diphosphate
-
 Abies grandis (-)-abietadiene + diphosphate
-
 ?
5.5.1.12 geranylgeranyl diphosphate
-
 Abies grandis (+)-copalyl diphosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.18 2
-
 copalyl diphosphate mutants D361A, D402A, D402E, D404N, D404A, D404E, D404N, D405A, D405E, D405N, D621A, E499A, R365A, R411A, R454A, W358A, Y520A Abies grandis
5.5.1.12 0.1
-
 geranylgeranyl diphosphate D405N, Y520A Abies grandis
5.5.1.12 0.2
-
 geranylgeranyl diphosphate D402N, R365A, R454A Abies grandis
5.5.1.12 0.5
-
 geranylgeranyl diphosphate R411A Abies grandis
5.5.1.12 2.2
-
 geranylgeranyl diphosphate
-
 Abies grandis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.18 8.7
-
 with copalyl diphosphate as substrate Abies grandis
5.5.1.12 7.2
-
 with geranylgeranyl diphosphate as substrate Abies grandis