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Literature summary extracted from
- Diterpenoid resin acid biosynthesis in conifers: enzymic cyclization of geranylgeranyl pyrophosphate to abietadiene, the precursor of abietic acid (1994), Arch. Biochem. Biophys., 313, 139-149.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.5.1.12 | diethyl dicarbonate | - |
Abies grandis |  |
| 5.5.1.12 | diethyl dicarbonate | - |
Pinus contorta | |
| 5.5.1.12 | Fe2+ | at concentrations about 0.1 mM | Abies grandis | |
| 5.5.1.12 | Fe2+ | at concentrations about 0.1 mM | Pinus contorta | |
| 5.5.1.12 | Mn2+ | at concentrations about 0.1 mM | Abies grandis | |
| 5.5.1.12 | Mn2+ | at concentrations about 0.1 mM | Pinus contorta | |
| 5.5.1.12 | p-hydroxymercuribenzoate | - |
Abies grandis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.5.1.12 | Fe2+ | - |
Abies grandis | |
| 5.5.1.12 | Fe2+ | - |
Pinus contorta | |
| 5.5.1.12 | Mg2+ | maximal activity at 2.5 mM | Abies grandis | |
| 5.5.1.12 | Mg2+ | maximal activity at 2.5 mM | Pinus contorta | |
| 5.5.1.12 | Mn2+ | - |
Abies grandis | |
| 5.5.1.12 | Mn2+ | - |
Pinus contorta | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.5.1.12 | 80000 | - |
gel filtration | Abies grandis |
| 5.5.1.12 | 80000 | - |
gel filtration | Pinus contorta |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.5.1.12 | geranylgeranyl diphosphate | Abies grandis | - |
(+)-copalyl diphosphate | - |
? | |
| 5.5.1.12 | geranylgeranyl diphosphate | Pinus contorta | - |
(+)-copalyl diphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.5.1.12 | Abies grandis | - |
- |
- |
| 5.5.1.12 | Pinus contorta | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.5.1.12 | - |
Abies grandis |
| 5.5.1.12 | - |
Pinus contorta |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.5.1.12 | 0.0214 | - |
- |
Abies grandis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.5.1.12 | geranylgeranyl diphosphate | - |
Abies grandis | (+)-copalyl diphosphate | - |
? | |
| 5.5.1.12 | geranylgeranyl diphosphate | - |
Pinus contorta | (+)-copalyl diphosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.5.1.12 | monomer | 1 * 80000, SDS-PAGE | Abies grandis |
| 5.5.1.12 | monomer | 1 * 80000, SDS-PAGE | Pinus contorta |
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