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Literature summary extracted from

  • Ochs, D.; Tappe, C.H.; Gaertner, P.; Kellner, R.; Poralla, K.
    Properties of purified squalene-hopene cyclase from Bacillus acidocaldarius (1990), Eur. J. Biochem., 194, 75-80.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.99.17 sodium taurodeoxycholate 1.5fold activation at 0.16% Alicyclobacillus acidocaldarius

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.17 (5-hydroxycarvacryl)trimethylammonium chloride 1-piperidine carboxylate i.e. AMO 1618, competitive inhibition Alicyclobacillus acidocaldarius
5.4.99.17 azasqualene inhibition at 0.001 mM Alicyclobacillus acidocaldarius
5.4.99.17 Cu2+ slight inhibition at 1 mM Alicyclobacillus acidocaldarius
5.4.99.17 dodecyldimethylamine N-oxide competitive inhibition Alicyclobacillus acidocaldarius
5.4.99.17 dodecyltrimethylammonium bromide competitive inhibition, 50% inhibition at 0.0001 mM Alicyclobacillus acidocaldarius
5.4.99.17 farnesol inhibition at 0.1 mM Alicyclobacillus acidocaldarius
5.4.99.17 Fe2+ slight inhibition at 1 mM Alicyclobacillus acidocaldarius
5.4.99.17 additional information inhibition by n-alkyldimethylammoniumhalides with alkyl chain lengths between 12 and 18 C atoms, inhibition increases with decreasing chain length Alicyclobacillus acidocaldarius
5.4.99.17 sodium dodecylsulfate strong inhibition Alicyclobacillus acidocaldarius
5.4.99.17 sodium taurodeoxycholate under 0.15% and above 0.25% Alicyclobacillus acidocaldarius
5.4.99.17 Zn2+ slight inhibition at 5 mM Alicyclobacillus acidocaldarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.17 0.003
-
 squalene
-
 Alicyclobacillus acidocaldarius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.4.99.17 cytoplasmic membrane
-
 Alicyclobacillus acidocaldarius
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.17 75000
-
 2 * 75000, SDS-PAGE, enzyme forms aggregates in absence of detergent Alicyclobacillus acidocaldarius
5.4.99.17 150000
-
 gel filtration Alicyclobacillus acidocaldarius

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.17 Alicyclobacillus acidocaldarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.17 homogeneity Alicyclobacillus acidocaldarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.17 0.345
-
-
 Alicyclobacillus acidocaldarius

Subunits

EC Number Subunits Comment Organism
5.4.99.17 dimer 2 * 75000, SDS-PAGE, enzyme forms aggregates in absence of detergent Alicyclobacillus acidocaldarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.17 60
-
-
 Alicyclobacillus acidocaldarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.17 6
-
-
 Alicyclobacillus acidocaldarius

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.4.99.17 0.00014
-
 dodecyldimethylamine N-oxide
-
 Alicyclobacillus acidocaldarius
5.4.99.17 0.00032
-
 dodecyltrimethylammonium bromide
-
 Alicyclobacillus acidocaldarius
5.4.99.17 0.00054
-
 (5-hydroxycarvacryl)trimethylammonium chloride 1-piperidine carboxylate
-
 Alicyclobacillus acidocaldarius