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Literature summary extracted from
- Purification and properties of the squalene-hopene cyclase from Rhodopseudomonas palustris, a purple non-sulfur bacterium producing hopanoids and tetrahymanol (1994), Biochim. Biophys. Acta, 1210, 317-320.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.17 | HECAMEG | 80% inactivation compared to CHAPS | Rhodopseudomonas palustris |  |
| 5.4.99.17 | octylthiogucopyranoside | complete inactivation | Rhodopseudomonas palustris | |
| 5.4.99.17 | taurodeoxycholate | 80% inactivation compared to CHAPS | Rhodopseudomonas palustris | |
| 5.4.99.17 | Triton-X100 | 96% inhibition | Rhodopseudomonas palustris | |
| 5.4.99.17 | Zwittergent | 80% inactivation compared to CHAPS | Rhodopseudomonas palustris |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.4.99.17 | membrane | - |
Rhodopseudomonas palustris | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.17 | 70000 | - |
SDS-PAGE | Rhodopseudomonas palustris |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.17 | Rhodopseudomonas palustris | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.17 | 2000-fold | Rhodopseudomonas palustris |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.17 | 0.1487 | - |
- |
Rhodopseudomonas palustris |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.4.99.17 | -20°C, stable for weeks | Rhodopseudomonas palustris |
| 5.4.99.17 | 6°C, stable for several days | Rhodopseudomonas palustris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.17 | squalene | - |
Rhodopseudomonas palustris | hop-22(29)-ene | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.17 | 30 | - |
- |
Rhodopseudomonas palustris |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.17 | 6.5 | - |
- |
Rhodopseudomonas palustris |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.17 | 5 | 8 | no activity below pH 5 or above pH 8 | Rhodopseudomonas palustris |
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