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Literature summary extracted from
- Site-directed mutagenesis of putative active-site residues in squalene-hopene cyclase (1996), Eur. J. Biochem., 242, 51-55.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.17 | - |
Alicyclobacillus acidocaldarius |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.17 | D376E | 10% enzyme activity | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | D376E/D377E | no enzyme activity | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | D376G | 0.2% activity when enzyme concentration is increased to 100fold | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | D376Q | no enzyme activity | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | D376R | no enzyme activity | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | D377E | 0.2% activity when enzyme concentration is increased to 100fold | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | D377G | 0.2% activity when enzyme concentration is increased to 100fold | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | D377Q | 0.2% activity when enzyme concentration is increased to 100fold | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | D377R | no enzyme activity | Alicyclobacillus acidocaldarius |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.17 | 0.017 | - |
squalene | wild type enzyme and D376E mutant | Alicyclobacillus acidocaldarius |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.17 | 72000 | - |
SDS-PAGE | Alicyclobacillus acidocaldarius |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.17 | Alicyclobacillus acidocaldarius | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.17 | wild type and mutants, homogeneity | Alicyclobacillus acidocaldarius |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.17 | additional information | - |
specific activities for wild type and mutant enzymes | Alicyclobacillus acidocaldarius |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.17 | 60 | - |
wild type enzyme: 50% loss of activity after 120 min, D376E mutant: 50% loss of activity after 100 min | Alicyclobacillus acidocaldarius |
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Release 2023.1 (January 2023)
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