BRENDA - Enzyme Database

Vellosimine reductase. A specific enzyme involved in the cell-free biosynthesis of sarpagine type alkaloids

Pfitzner, A.; Stoeckigt, J.; Tetrahedron Lett. 24, 1695-1698 (1983)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.273
0.0167
-
vellosimine
-
Rauvolfia serpentina
1.1.1.273
0.02
-
NADPH
-
Rauvolfia serpentina
1.1.1.273
0.11
-
geissoschizal
-
Rauvolfia serpentina
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.273
42000
-
gel filtration
Rauvolfia serpentina
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.273
vellosimine + NADPH
Rauvolfia serpentina
specific for NADPH, may be involved in the conversion of sarpagine type alkaloids
10-deoxysarpagine + NADP+
-
Rauvolfia serpentina
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.273
Rauvolfia serpentina
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.273
DEAE-cellulose, hydroxylapatite, Matrex gel Green A
Rauvolfia serpentina
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.273
cell suspension culture
-
Rauvolfia serpentina
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.273
0.00096
-
geissoschizal reduction
Rauvolfia serpentina
1.1.1.273
0.0012
-
vellosimine reduction
Rauvolfia serpentina
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.273
geissoschizal + NADPH
-
440037
Rauvolfia serpentina
?
-
-
-
?
1.1.1.273
vellosimine + NADPH
specific for NADPH
440037
Rauvolfia serpentina
10-deoxysarpagine + NADP+
-
440037
Rauvolfia serpentina
?
1.1.1.273
vellosimine + NADPH
specific for NADPH, may be involved in the conversion of sarpagine type alkaloids
440037
Rauvolfia serpentina
10-deoxysarpagine + NADP+
-
440037
Rauvolfia serpentina
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.273
7.5
-
-
Rauvolfia serpentina
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.273
NADPH
-
Rauvolfia serpentina
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.273
NADPH
-
Rauvolfia serpentina
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.273
0.0167
-
vellosimine
-
Rauvolfia serpentina
1.1.1.273
0.02
-
NADPH
-
Rauvolfia serpentina
1.1.1.273
0.11
-
geissoschizal
-
Rauvolfia serpentina
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.273
42000
-
gel filtration
Rauvolfia serpentina
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.273
vellosimine + NADPH
Rauvolfia serpentina
specific for NADPH, may be involved in the conversion of sarpagine type alkaloids
10-deoxysarpagine + NADP+
-
Rauvolfia serpentina
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.273
DEAE-cellulose, hydroxylapatite, Matrex gel Green A
Rauvolfia serpentina
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.273
cell suspension culture
-
Rauvolfia serpentina
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.273
0.00096
-
geissoschizal reduction
Rauvolfia serpentina
1.1.1.273
0.0012
-
vellosimine reduction
Rauvolfia serpentina
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.273
geissoschizal + NADPH
-
440037
Rauvolfia serpentina
?
-
-
-
?
1.1.1.273
vellosimine + NADPH
specific for NADPH
440037
Rauvolfia serpentina
10-deoxysarpagine + NADP+
-
440037
Rauvolfia serpentina
?
1.1.1.273
vellosimine + NADPH
specific for NADPH, may be involved in the conversion of sarpagine type alkaloids
440037
Rauvolfia serpentina
10-deoxysarpagine + NADP+
-
440037
Rauvolfia serpentina
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.273
7.5
-
-
Rauvolfia serpentina