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Literature summary extracted from

  • Seong, B.L.; Son, H.J.; Mheen, T.I.; Park, Y.H.; Han, M.H.
    Enzymatic oxidation of rifamycins by a microorganism of the genus Humicola (1985), J. Ferment. Technol., 63, 515-522.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.10.3.6 medicine rifamycin, an ansa-macrolide antibiotic, is used for production of antituberculosic agents Humicola spp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.6 Fe2+
-
 Humicola spp.
1.10.3.6 Hg2+
-
 Humicola spp.
1.10.3.6 Rifamycin B concentration above 2 mM Humicola spp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.6 0.05
-
 Rifamycin B
-
 Humicola spp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.6 intracellular
-
 Humicola spp. 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.6 additional information not affected by other metal ions than Fe2+, Hg2+ Humicola spp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.6 rifamycin B + O2 Humicola spp.
-
 rifamycin O + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.6 Humicola spp.
-
 ATCC 20620
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.6 partially, ammonium sulfate precipitation Humicola spp.

Reaction

EC Number Reaction Comment Organism Reaction ID
1.10.3.6 rifamycin B + O2 = rifamycin O + H2O2 acts on various kinds of diphenol compounds, converting them into quinonic forms and H2O2 Humicola spp.
a

Storage Stability

EC Number Storage Stability Organism
1.10.3.6 4°C, 100 mM phosphate buffer, pH 7.8, one month, no appreciable loss of activity Humicola spp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.6 catechol + O2
-
 Humicola spp. ?
-
 ?
1.10.3.6 p-hydroquinone + O2
-
 Humicola spp. ?
-
 ?
1.10.3.6 p-hydroxyphenoxyacetic acid + O2
-
 Humicola spp. ?
-
 ?
1.10.3.6 rifamycin B + O2
-
 Humicola spp. rifamycin O + H2O2
-
 ?
1.10.3.6 rifamycin B + O2 most specific, highest catalytic efficiency Humicola spp. rifamycin O + H2O2
-
 ?
1.10.3.6 rifamycin SV + ?
-
 Humicola spp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.6 45
-
 above the catalytic activity is greatly reduced Humicola spp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.6 7.8 8
-
 Humicola spp.