Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • De Leonardis, S.; Dipierro, N.; Dipierro, S.
    Purification and characterization of an ascorbate peroxidase from potato tuber mitochondria (2000), Plant Physiol. Biochem., 38, 773-779.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.11.1.11 the enzyme is completely inactivated within 30 s in ascorbate-depleted medium under anaerobic conditions Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.11 cysteine 100% inhibition at 5 mM Solanum tuberosum
1.11.1.11 iodoacetamide 30% inhibition at 1 mM, 65% inhibition at 5 mM Solanum tuberosum
1.11.1.11 KCN 100% inhibition at 0.5 mM Solanum tuberosum
1.11.1.11 Mersalyl 58% inhibition at 0.005 mM, 100% inhibition at 0.05 mM Solanum tuberosum
1.11.1.11 N-ethylmaleimide 33% inhibition at 0.05 mM, 28% inhibition at 0.5 mM Solanum tuberosum
1.11.1.11 NaN3 80% inhibition at 1 mM Solanum tuberosum
1.11.1.11 p-hydroxymercuribenzoate 43% inhibition at 0.005 mM, 100% inhibition at 0.05 mM Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.076
-
 ascorbate
-
 Solanum tuberosum
1.11.1.11 0.08
-
 H2O2
-
 Solanum tuberosum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.11 mitochondrion inside, either bound to the inner membrane or in the matrix Solanum tuberosum 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.11 31000
-
 x * 31000, SDS-PAGE Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.11 L-ascorbate + H2O2 Solanum tuberosum role of the mitochondrial enzyme in the scanvenging of toxic oxygen species inside potato tuber mitochondria dehydroascorbate + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.11 using lysed mitochondria, and column chromatography on DEAE-Sephacel, Sephadex G-75, DE-52 and hydroxylapatite Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.11 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.11 36
-
 purified enzyme Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 guaiacol + H2O2 the activity is lower than with L-ascorbate Solanum tuberosum ?
-
 ?
1.11.1.11 L-ascorbate + H2O2 highly specific for Solanum tuberosum dehydroascorbate + H2O
-
 ?
1.11.1.11 L-ascorbate + H2O2 role of the mitochondrial enzyme in the scanvenging of toxic oxygen species inside potato tuber mitochondria Solanum tuberosum dehydroascorbate + 2 H2O
-
 ?
1.11.1.11 pyrogallol + H2O2 the activity is lower than with L-ascorbate Solanum tuberosum 3-hydroxybenzo-1,2-quinone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.11 ? x * 31000, SDS-PAGE Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.11 7
-
-
 Solanum tuberosum