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Literature summary extracted from

  • Takeda, T.; Yoshimura, K.; Ishikawa, T.; Shigeoka, S.
    Purification and characterization of ascorbate peroxidase in Chlorella vulgaris (1998), Biochimie, 80, 295-301.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.11.1.11 the half-inactivation time is estimated to be 15 min, when the enzyme is diluted with ascorbate-depleted medium Chlorella vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.11 KCN strong inhibition at 1 mM Chlorella vulgaris
1.11.1.11 NaN3 strong inhibition at 5 mM Chlorella vulgaris
1.11.1.11 p-chloromercuribenzoate 82% inhibition at 0.2 mM for 5 min Chlorella vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.02
-
 H2O2
-
 Chlorella vulgaris
1.11.1.11 0.111
-
 ascorbate
-
 Chlorella vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.11 32000
-
 gel filtration Chlorella vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Chlorella vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.11 using ultra centrifugation, column chromatography on DEAE-Sephacel, ammonium sulfate precipitation, column chromatography on phenyl Sepharose, ammonium sulfate precipitation and column chromatography on Superdex 200 and Phenyl Superose Chlorella vulgaris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.11 3.3
-
 cell extract Chlorella vulgaris
1.11.1.11 1307
-
 purified enzyme Chlorella vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 cytochrome c + H2O2 no activity Chlorella vulgaris ? + H2O
-
 ?
1.11.1.11 glutathione + H2O2 no activity Chlorella vulgaris ? + H2O
-
 ?
1.11.1.11 guaiacol + H2O2 no activity Chlorella vulgaris ?
-
 ?
1.11.1.11 L-ascorbate + H2O2
-
 Chlorella vulgaris dehydroascorbate + H2O
-
 ?
1.11.1.11 L-ascorbic acid + cumene hydroperoxide no activity Chlorella vulgaris dehydroascorbate + 1,1-dimethylbenzylalcohol + H2O
-
 ?
1.11.1.11 L-ascorbic acid + tert-butylhydroperoxide no activity Chlorella vulgaris dehydroascorbate + tert-butylalcohol
-
 ?
1.11.1.11 additional information no activity with: glutathione, cytochrome c, NADH and NADPH Chlorella vulgaris ?
-
 ?
1.11.1.11 NADPH + H2O2 no activity Chlorella vulgaris ? + H2O
-
 ?
1.11.1.11 pyrogallol + H2O2 62.6% activity relative to L-ascorbate Chlorella vulgaris 3-hydroxybenzo-1,2-quinone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.11 monomer 1 * 32000, SDS-PAGE Chlorella vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.11 36.4
-
-
 Chlorella vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.11 50
-
 the enzyme retains full activity between pH 6 and pH 7.5 Chlorella vulgaris
1.11.1.11 60
-
 complete loss of activity Chlorella vulgaris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.11 696
-
 ascorbate
-
 Chlorella vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.11 6.1
-
-
 Chlorella vulgaris
1.11.1.11 7
-
 assay at Chlorella vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.11 heme
-
 Chlorella vulgaris