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Literature summary extracted from

  • Ohya, T.; Morimura, Y.; Saji, H.; Mihara, T.; Ikawa, T.
    Purification and characterization of ascorbate peroxidase in roots of Japanese radish (1997), Plant Sci., 125, 137-145.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.11.1.11 dialysis of crude enzyme preparations for 24 h against a buffer depleted of ascorbate or sorbitol results in 90% and 20% loss in enzyme activity, respectively. In air saturated solutions, salting-out results in a significant loss in enzyme activity, therefore exchange to N2 gas is performed prior to salting-out of the root enzyme Raphanus sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.11 2-mercaptoethanol not inhibitory at 0.5 mM, 31% inhibition at 5 mM Raphanus sativus
1.11.1.11 5,5'-dithiobis(2-nitrobenzoic acid) 40% inhibition at 0.1 mM Raphanus sativus
1.11.1.11 cysteine 50% inhibition at 5 mM Raphanus sativus
1.11.1.11 dithioerythritol 67% inhibition at 0.05 mM Raphanus sativus
1.11.1.11 dithiothreitol 54% inhibition at 0.05 mM Raphanus sativus
1.11.1.11 EDTA not inhibitory Raphanus sativus
1.11.1.11 iodoacetate not inhibitory Raphanus sativus
1.11.1.11 KCN 95% inhibition at 0.1 mM Raphanus sativus
1.11.1.11 NaN3 17% inhibition at 1 mM, 87% inhibition at 10 mM Raphanus sativus
1.11.1.11 p-chloromercuribenzoate 87% inhibition at 0.005 mM, inactivation is partially reversible, 2-mercaptoethanol protects Raphanus sativus
1.11.1.11 reduced glutathione 33% inhibition at 5 mM Raphanus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.13
-
 H2O2
-
 Raphanus sativus
1.11.1.11 0.77
-
 ascorbate
-
 Raphanus sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.11 cytosol
-
 Raphanus sativus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.11 Fe
-
 Raphanus sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.11 28000
-
 native PAGE Raphanus sativus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Raphanus sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.11 using ammonium sulfate precipitation, dialysis and column chromatography on butyl-Toyopearl, DEAE-cellulofine and Sephadex G-75 Raphanus sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.11 root
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.11 561.1
-
 purified enzyme Raphanus sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 cytochrome c + H2O2 no activity Raphanus sativus ? + H2O
-
 ?
1.11.1.11 guaiacol + H2O2 no activity Raphanus sativus ?
-
 ?
1.11.1.11 L-ascorbate + H2O2 highly specific for L-ascorbate Raphanus sativus dehydroascorbate + H2O
-
 ?
1.11.1.11 additional information no activity with: cytochrome c, reduced glutathione, NADH, NADPH, 6-palmityl-ascorbate, ascorbate-2-sulfate, guaiacol, 3,3'-diaminobenzidine, pyrocatechol or D-iso-ascorbate Raphanus sativus ?
-
 ?
1.11.1.11 o-dianisidine + H2O2 the oxidation rate is only 8.6% of that with L-ascorbate Raphanus sativus ?
-
 ?
1.11.1.11 pyrogallol + H2O2 the oxidation rate is only 5.5% of that with L-ascorbate Raphanus sativus 3-hydroxybenzo-1,2-quinone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.11 monomer 1 * 28000, SDS-PAGE Raphanus sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.11 6
-
-
 Raphanus sativus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.11 heme
-
 Raphanus sativus