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Literature summary extracted from

  • Ishikawa, T.; Takeda, T.; Shigeoka, S.
    Purification and characterization of cytosolic ascorbate peroxidase from komatsuna (Brassica rapa) (1996), Plant Sci., 120, 11-18.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.11 KCN complete inhibition at 0.05 mM Brassica rapa
1.11.1.11 NaN3 complete inhibition at 1 mM Brassica rapa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.024
-
 H2O2
-
 Brassica rapa
1.11.1.11 0.402
-
 ascorbate
-
 Brassica rapa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.11 chloroplast stroma
-
 Brassica rapa 9570
-
1.11.1.11 cytosol
-
 Brassica rapa 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.11 28000
-
 gel filtration Brassica rapa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.11 L-ascorbate + H2O2 Brassica rapa
-
 dehydroascorbate + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Brassica rapa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.11 using column chromatography on DEAE-Sephacel, ammonium sulfate treatment and column chromatography on butyl-Toyopearl and TSK G3000SW Brassica rapa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.11 leaf
-
 Brassica rapa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.11 56
-
 purified enzyme Brassica rapa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 cytochrome c + H2O2 no activity Brassica rapa ? + H2O
-
 ?
1.11.1.11 glutathione + H2O2 no activity Brassica rapa ? + H2O
-
 ?
1.11.1.11 guaiacol + H2O2 no reaction Brassica rapa ?
-
 ?
1.11.1.11 L-ascorbate + H2O2 L-ascorbate is the most effective natural electron donor Brassica rapa dehydroascorbate + H2O
-
 ?
1.11.1.11 L-ascorbate + H2O2
-
 Brassica rapa dehydroascorbate + 2 H2O
-
 ?
1.11.1.11 additional information the cytosolic enzyme exhibits no activity with: glutathione, cytochrome c and NAD(P)H Brassica rapa ?
-
 ?
1.11.1.11 NADPH + H2O2 no activity Brassica rapa ? + H2O
-
 ?
1.11.1.11 pyrogallol + H2O2 2.5-fold higher rate than that of L-ascorbate Brassica rapa 3-hydroxybenzo-1,2-quinone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.11 monomer 1 * 28000, SDS-PAGE Brassica rapa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.11 38
-
-
 Brassica rapa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.11 55
-
 complete loss of activity Brassica rapa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.11 6.3
-
 assay at Brassica rapa
1.11.1.11 6.5
-
-
 Brassica rapa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.11 6.5 7.5
-
 Brassica rapa

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.11 heme
-
 Brassica rapa