EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | diphosphate | stimulates by chelating and stabilizing Mn3+ | Lentinula edodes | |
1.11.1.13 | dithiothreitol | greatly enhances the oxidation of verartryl alcohol, lignin-model compounds and lignin | Lentinula edodes | |
1.11.1.13 | glutathione | greatly enhances the oxidation of verartryl alcohol, lignin-model compounds and lignin | Lentinula edodes | |
1.11.1.13 | H2O2 | H2O2-dependent | Lentinula edodes | |
1.11.1.13 | L-malate | activates by chelating and stabilizing Mn3+ | Lentinula edodes | |
1.11.1.13 | L-Tartrate | stimulates by chelating and stabilizing Mn3+ | Lentinula edodes | |
1.11.1.13 | malonate | stimulates by chelating and stabilizing Mn3+ | Lentinula edodes | |
1.11.1.13 | methylmalonate | stimulates by chelating and stabilizing Mn3+ | Lentinula edodes | |
1.11.1.13 | additional information | no Mn3+ chelators and activators: acetate, propionate, citrate, D-malate, ethylene glycol | Lentinula edodes | |
1.11.1.13 | additional information | succinate is no Mn3+ chelator and activator | Lentinula edodes | |
1.11.1.13 | oxalate | activates by chelating and stabilizing Mn3+ | Lentinula edodes | |
1.11.1.13 | oxaloacetate | stimulates by chelating and stabilizing Mn3+ | Lentinula edodes |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | additional information | not inhibited by 0.1 mM Cu2+, Co2+ or salicylic acid; not inhibited by salicylic acid | Lentinula edodes |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.11.1.13 | extracellular | - |
Lentinula edodes | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 44600 | - |
x * 44600, SDS-PAGE | Lentinula edodes |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.13 | Lentinula edodes | - |
syn. Lentinus edodes, heterodikaryon strain 861 | - |
1.11.1.13 | Lentinula edodes | - |
white rot basidomycete, produces edible shiitake mushroom | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.13 | MnP1: 19fold purification | Lentinula edodes |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 660 | - |
o-toluidine as substrate for Mn3+-oxidation | Lentinula edodes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.13 | Mn2+ + H+ + H2O2 | - |
Lentinula edodes | Mn3+ + H2O | chelating organic acids facilitate the dissociation of Mn3+ from enzyme | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | - |
Lentinula edodes | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | - |
Lentinula edodes | Mn3+ + H2O | Mn3+ oxidizes vanillylacetone | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | - |
Lentinula edodes | Mn3+ + H2O | chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential | ? | |
1.11.1.13 | additional information | enzyme is able to oxidatively depolymerize both dimeric lignin-model compounds and milled spruce-wood lignin | Lentinula edodes | ? | - |
? | |
1.11.1.13 | additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Lentinula edodes | ? | - |
? | |
1.11.1.13 | additional information | enzyme oxidizes veratryl alcohol and o-tolidine | Lentinula edodes | ? | - |
? | |
1.11.1.13 | additional information | in presence of H2O2 and Mn2+ the enzyme oxidizes lignin and lignin-model compounds | Lentinula edodes | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.11.1.13 | ? | x * 44600, SDS-PAGE | Lentinula edodes |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 40 | - |
assay at | Lentinula edodes |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 20 | 65 | progressive enhancement of activity from 20°C to 65°C | Lentinula edodes |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 100 | - |
complete inactivation after boiling for 5 min | Lentinula edodes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | additional information | - |
pI: 3.2 | Lentinula edodes |
1.11.1.13 | 3.5 | 5.5 | - |
Lentinula edodes |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | heme | heme-iron protein | Lentinula edodes | |
1.11.1.13 | heme | one iron protoporphyrin IX prosthetic group per enzyme molecule | Lentinula edodes |