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Literature summary extracted from
- Purification of the o-dianisidine peroxidase from Escherichia coli B: Physicochemical characterization and analysis of its dual catalatic and peroxidatic activities (1979), J. Biol. Chem., 254, 4245-4252.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.6 | histidine | stimulation | Escherichia coli |  |
| 1.11.1.6 | imidazole | 2fold activation at 10 mM | Escherichia coli | |
| 1.11.1.6 | Tris | stimulation at 10 mM | Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.6 | 3.9 | - |
H2O2 | - |
Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | 84000 | - |
4 * 84000, SDS-PAGE | Escherichia coli |
| 1.11.1.6 | 337000 | - |
sedimentation equillibrium analysis | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.6 | H2O2 | Escherichia coli | bifunctional catalase-peroxidase | O2 + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.6 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.1.6 | to homogeneity, chromatography steps | Escherichia coli |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.11.1.6 | cell culture | - |
Escherichia coli | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | 15.2 | - |
peroxidatic activity | Escherichia coli |
| 1.11.1.6 | 1486 | - |
catalatic activity | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.6 | H2O2 | bifunctional catalase-peroxidase | Escherichia coli | O2 + H2O | - |
? | |
| 1.11.1.6 | o-dianisidine + H2O2 | peroxidatic activity | Escherichia coli | ? + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.11.1.6 | tetramer | 4 * 84000, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.6 | 16300 | - |
H2O2 | - |
Escherichia coli | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | 6.5 | - |
peroxidatic activity | Escherichia coli |
| 1.11.1.6 | 7.5 | - |
catalatic activity | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.6 | heme | 2.07 molecules protoheme IX per tetramer | Escherichia coli | |
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