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Literature summary extracted from

  • Terzenbach, D.P.; Blaut, M.
    Purification and characterization of a catalase from the nonsulfur phototrophic bacterium Rhodobacter sphaeroides ATH 2.4.1 and its role in the oxidative stress response (1998), Arch. Microbiol., 169, 503-508.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.11.1.6 resistant up to 7 M urea, 10% SDS Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 2-mercaptoethanol 36% inhibition at 1 mM Cereibacter sphaeroides
1.11.1.6 hydroxylamine 85.1% inactivation at 0.005 mM Cereibacter sphaeroides
1.11.1.6 KCN 72.5% inactivation at 0.005 mM Cereibacter sphaeroides
1.11.1.6 NaN3 76.4% inactivation at 0.005 mM Cereibacter sphaeroides
1.11.1.6 Sodium dithionite inhibitory Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 40
-
 H2O2
-
 Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 68000
-
 1 * 75000 + 1 * 68000, SDS-PAGE Cereibacter sphaeroides
1.11.1.6 75000
-
 1 * 75000 + 1 * 68000, SDS-PAGE Cereibacter sphaeroides
1.11.1.6 150000
-
 native PAGE Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 H2O2 Cereibacter sphaeroides no peroxidase-activity O2 + H2O
-
 ?
1.11.1.6 H2O2 Cereibacter sphaeroides monofunctional catalase O2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 to homogeneity, chromatography techniques Cereibacter sphaeroides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.6 cell culture inducible by H2O2, heat-shock, ethanol and stationary-phase conditions Cereibacter sphaeroides
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 55930
-
-
 Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
1.11.1.6 4°C, 90% of the activity lost in 14 days Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2 no peroxidase-activity Cereibacter sphaeroides O2 + H2O
-
 ?
1.11.1.6 H2O2 monofunctional catalase Cereibacter sphaeroides O2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 dimer 1 * 75000 + 1 * 68000, SDS-PAGE Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.6 54
-
-
 Cereibacter sphaeroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.6 30
-
 50% loss of activity in 30 min Cereibacter sphaeroides
1.11.1.6 80
-
 complete loss of activity Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 6 10
-
 Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.6 heme protoheme IX Cereibacter sphaeroides