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Literature summary extracted from

  • Popov, V.O.; Gazaryan, I.G.; Egorov, A.M.; Berezin, I.V.
    NAD-dependent hydrogenase from the hydrogen-oxidizing bacterium Alcaligenes eutrophum Z1. Kinetic studies of the NADH-dehydrogenase activity (1985), Biochim. Biophys. Acta, 827, 466-471.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.1.2 0.013
-
 NADH electron acceptor resazurine Cupriavidus necator
1.12.1.2 0.028
-
 oxidized resazurin
-
 Cupriavidus necator
1.12.1.2 0.04
-
 NADH electron acceptor benzyl viologen Cupriavidus necator
1.12.1.2 0.042
-
 NADH electron acceptor methyl viologen Cupriavidus necator
1.12.1.2 0.054
-
 NADH electron acceptor ferricyanide Cupriavidus necator
1.12.1.2 0.06
-
 NADH electron acceptor 2,6-dichlorophenolindophenol Cupriavidus necator
1.12.1.2 0.07
-
 NADH electron acceptor methylene blue Cupriavidus necator
1.12.1.2 0.25
-
 oxidized methylene blue
-
 Cupriavidus necator
1.12.1.2 0.44
-
 oxidized 2,6-dichlorophenolindophenol
-
 Cupriavidus necator
1.12.1.2 0.45
-
 ferricyanide
-
 Cupriavidus necator
1.12.1.2 4.1
-
 benzyl viologen
-
 Cupriavidus necator
1.12.1.2 5.9
-
 methyl viologen
-
 Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Cupriavidus necator
-
-
-
1.12.1.2 Cupriavidus necator
-
 formerly Alcaligenes eutrophus
-
1.12.1.2 Cupriavidus necator Z1
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.12.1.2 H2 + NAD+ = H+ + NADH ping pong mechanism Cupriavidus necator
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.1.2 30 40
-
 Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 H2 + NAD+ artificial electron acceptor resazurin Cupriavidus necator H+ + NADH
-
 ?
1.12.1.2 H2 + NAD+ artificial electron acceptor resazurin Cupriavidus necator Z1 H+ + NADH
-
 ?
1.12.1.2 oxidized 2,6-dichlorophenol indophenol + NADH + H+
-
 Cupriavidus necator reduced 2,6-dichlorophenolindophenol + NAD+
-
 r
1.12.1.2 oxidized 2,6-dichlorophenol indophenol + NADH + H+
-
 Cupriavidus necator Z1 reduced 2,6-dichlorophenolindophenol + NAD+
-
 r
1.12.1.2 oxidized methylene blue + NADH + H+
-
 Cupriavidus necator reduced methylene blue + NAD+
-
 r
1.12.1.2 oxidized methylene blue + NADH + H+
-
 Cupriavidus necator Z1 reduced methylene blue + NAD+
-
 r
1.12.1.2 oxidized resazurin + NADH + H+
-
 Cupriavidus necator reduced resazurin + NAD+
-
 r
1.12.1.2 oxidized resazurin + NADH + H+
-
 Cupriavidus necator Z1 reduced resazurin + NAD+
-
 r