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Literature summary extracted from

  • Pfitzner, J.; Linke, H.A.B.; Schlegel, H.G.
    Properties of the NAD-specific hydrogenase from Hydrogenomonas H 16 (1970), Arch. Mikrobiol., 71, 67-78.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.1.2 mercaptoethanol
-
 Hydrogenomonas sp.
1.12.1.2 Sulfide
-
 Hydrogenomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.1.2 0.13
-
 NAD+
-
 Hydrogenomonas sp.
1.12.1.2 0.19
-
 H2
-
 Hydrogenomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.1.2 soluble
-
 Hydrogenomonas sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.1.2 Iron Fe2+ stabilizes Hydrogenomonas sp.
1.12.1.2 Mg2+ stabilizes Hydrogenomonas sp.
1.12.1.2 Mn2+ stabilizes Hydrogenomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Hydrogenomonas sp.
-
-
-
1.12.1.2 Hydrogenomonas sp. H16
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.1.2
-
 Hydrogenomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.1.2 36.5
-
-
 Hydrogenomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.12.1.2 -16°C, 10 d, 30% loss of activity Hydrogenomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 H2 + NAD+ electron acceptor FAD, only in presence of catalytic amounts of NAD+ Hydrogenomonas sp. H+ + NADH
-
 ?
1.12.1.2 H2 + NAD+ artificial electron acceptor methylene blue, only in presence of catalytic amounts of NAD+ Hydrogenomonas sp. H+ + NADH
-
 ?
1.12.1.2 H2 + NAD+ electron acceptor FMN, only in presence of catalytic amounts of NAD+ Hydrogenomonas sp. H+ + NADH
-
 ?
1.12.1.2 H2 + NAD+ electron acceptor FAD, only in presence of catalytic amounts of NAD+ Hydrogenomonas sp. H16 H+ + NADH
-
 ?
1.12.1.2 H2 + NAD+ artificial electron acceptor methylene blue, only in presence of catalytic amounts of NAD+ Hydrogenomonas sp. H16 H+ + NADH
-
 ?
1.12.1.2 H2 + NAD+ electron acceptor FMN, only in presence of catalytic amounts of NAD+ Hydrogenomonas sp. H16 H+ + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.1.2 36
-
-
 Hydrogenomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.1.2 7.9
-
 potassium phosphate buffer Hydrogenomonas sp.
1.12.1.2 8.5
-
 Tris-HCl buffer Hydrogenomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.12.1.2 6.9 9.1 Tris-HCl buffer and potassium phosphate buffer pH 6.9: 20% activity, Tris-HCl buffer pH 9.1: 40% activity, potassium phosphate buffer pH 9.1: 65% activity Hydrogenomonas sp.