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Literature summary extracted from

  • Ma, K.; Schicho, R.N.; Kelly, R.M.; Adams, M.W.W.
    Hydrogenase of the hyperthermophile Pyrococcus furiosus is an elemental sulfur reductase or sulfhydrogenase: Evidence for a sulfur-reducing hydrogenase ancestor (1993), Proc. Natl. Acad. Sci. USA, 90, 5341-5344.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.12.7.2 rubredoxin 3fold increase of sulfur reductase activity at pH 7.6 Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Pyrococcus furiosus
-
 strain DSM 3638
-
1.12.98.4 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2
-
 Pyrococcus furiosus
1.12.98.4 Q Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 S + NADPH
-
 Pyrococcus furiosus H2S + NADP+
-
 ?
1.12.98.4 H2 + polysulfide(n)
-
 Pyrococcus furiosus H2S + polysulfide(n-1)
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.12.98.4 sulfhydrogenase bifunctional enzyme using both elementar sulfur and polysulfide as substrates for H2S production Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.7.2 80
-
 sulfur reductase activity Pyrococcus furiosus
1.12.7.2 95
-
 above 95°C, hydrogen oxidation Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.7.2 8
-
 hydrogen oxidation Pyrococcus furiosus
1.12.7.2 8.4
-
 sulfur reductase activity Pyrococcus furiosus