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Literature summary extracted from

  • Ueno, Y.; Kurano, N.; Miyachi, S.
    Purification and characterization of hydrogenase from the marine green alga, Chlorococcum littorale (1999), FEBS Lett., 443, 144-148.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.7.2 O2 95% activity loss, few min at 4°C Alvikia littoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.7.2 0.00068
-
 oxidized ferredoxin
-
 Alvikia littoralis
1.12.7.2 0.5
-
 oxidized methyl viologen
-
 Alvikia littoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 55000
-
 SDS-PAGE Alvikia littoralis
1.12.7.2 57000
-
 gel filtration Alvikia littoralis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin Alvikia littoralis
-
 H+ + reduced ferredoxin
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Alvikia littoralis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2 partially Alvikia littoralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 75.7
-
 hydrogen evolution, reduced methyl viologen as electron donor Alvikia littoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin
-
 Alvikia littoralis H+ + reduced ferredoxin
-
 r
1.12.7.2 H2 + oxidized methyl viologen
-
 Alvikia littoralis H+ + reduced methyl viologen
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.7.2 50
-
-
 Alvikia littoralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.7.2 7.5
-
-
 Alvikia littoralis