EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.12.7.2 | O2 | 50% loss of H2 evolution activity after 3 h | Pyrococcus furiosus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.12.7.2 | membrane | - |
Pyrococcus furiosus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.12.7.2 | Fe | 4 Fe-atoms per mol hydrogenase | Pyrococcus furiosus | |
1.12.7.2 | Ni | 1 Ni atom per mol, redox active Ni-site | Pyrococcus furiosus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.12.7.2 | 20000 | - |
alpha, beta, 1 * 40000 + 1 * 20000, SDS-PAGE | Pyrococcus furiosus |
1.12.7.2 | 40000 | - |
alpha, beta, 1 * 40000 + 1 * 20000, SDS-PAGE | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.7.2 | Pyrococcus furiosus | - |
strain DSM 3638 | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.12.7.2 | rapid inactivation under aerobic conditions | Pyrococcus furiosus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.7.2 | under anaerobic conditions, solubilized with n-dodecyl-beta-D-maltoside | Pyrococcus furiosus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.12.7.2 | 0.004 | - |
hydrogen oxidation, benzyl viologen as electron acceptor | Pyrococcus furiosus |
1.12.7.2 | 9.4 | - |
hydrogen evolution, reduced methyl viologen as electron donor | Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.7.2 | H2 + electron acceptor | methyl viologen as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
1.12.7.2 | H2 + electron acceptor | benzyl viologen as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
1.12.7.2 | H2 + electron acceptor | NADP+ as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
1.12.7.2 | additional information | ferredoxin is not an efficient electron carrier for both hydrogenases | Pyrococcus furiosus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.7.2 | dimer | alpha, beta, 1 * 40000 + 1 * 20000, SDS-PAGE | Pyrococcus furiosus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.7.2 | 100 | - |
50% inactivation after 30 min | Pyrococcus furiosus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.12.7.2 | NADPH | - |
Pyrococcus furiosus |