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Literature summary extracted from

  • Meuer, J.; Bartoschek, S.; Koch, J.; Kunkel, A.; Hedderich, R.
    Purification and catalytic properties of Ech hydrogenase from Methanosarcina barkeri (1999), Eur. J. Biochem., 265, 325-335.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.7.2 O2 90% inactivation after 30 min Methanosarcina barkeri
1.12.99.6 CO 50% inhibition at 7.5% CO in the gas phase Methanosarcina barkeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.7.2 0.001
-
 Ferredoxin
-
 Methanosarcina barkeri
1.12.7.2 0.005
-
 H2
-
 Methanosarcina barkeri
1.12.7.2 0.0075
-
 reduced ferredoxin
-
 Methanosarcina barkeri
1.12.99.6 0.001
-
 oxidized ferredoxin
-
 Methanosarcina barkeri
1.12.99.6 0.005
-
 H2
-
 Methanosarcina barkeri
1.12.99.6 0.0075
-
 reduced ferredoxin
-
 Methanosarcina barkeri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.7.2 membrane
-
 Methanosarcina barkeri 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe 11.3 mol non-heme iron per mol enzyme Methanosarcina barkeri
1.12.7.2 Ni 0.9 mol Ni per mol enzyme Methanosarcina barkeri
1.12.99.6 Fe 11.3 mol per mol of enzyme Methanosarcina barkeri
1.12.99.6 Ni 0.9 mol per mol of enzyme Methanosarcina barkeri
1.12.99.6 Sulfide 10.8 mol acid labile sulfur per mol of enzyme Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.3 35000
-
 x * 57000 + x * 35000, SDS-PAGE Methanosarcina barkeri
1.12.98.3 57000
-
 x * 57000 + x * 35000, SDS-PAGE Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin Methanosarcina barkeri
-
 H+ + reduced ferredoxin
-
 r
1.12.7.2 additional information Methanosarcina barkeri major membrane protein in acetate-grown, methanol-grown or H2/CO2-grown cells ?
-
 ?
1.12.7.2 additional information Methanosarcina barkeri Fusaro / DSM 804 major membrane protein in acetate-grown, methanol-grown or H2/CO2-grown cells ?
-
 ?
1.12.99.6 H2 + acceptor Methanosarcina barkeri ferredoxin acts as physiological electron donor H+ + reduced acceptor
-
 ?
1.12.99.6 H2 + acceptor Methanosarcina barkeri Fusaro / DSM 804 ferredoxin acts as physiological electron donor H+ + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Methanosarcina barkeri
-
-
-
1.12.7.2 Methanosarcina barkeri Fusaro / DSM 804
-
-
-
1.12.98.3 Methanosarcina barkeri
-
-
-
1.12.99.6 Methanosarcina barkeri
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.7.2 rapid inactivation under aerobic conditions Methanosarcina barkeri

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2
-
 Methanosarcina barkeri
1.12.98.3
-
 Methanosarcina barkeri
1.12.99.6 apparent homogeneity Methanosarcina barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 50
-
 hydrogen oxidation, ferredoxin as electron acceptor Methanosarcina barkeri
1.12.7.2 53
-
 hydrogen oxidation, methyl viologen as electron acceptor Methanosarcina barkeri
1.12.7.2 90
-
 hydrogen evolution, ferredoxin as electron donor Methanosarcina barkeri
1.12.7.2 123
-
 hydrogen oxidation, methylene blue as electron acceptor Methanosarcina barkeri
1.12.7.2 210
-
 hydrogen oxidation, benzyl viologen as electron acceptor Methanosarcina barkeri
1.12.99.6 50
-
 reduction of ferredoxin Methanosarcina barkeri
1.12.99.6 90
-
 H2 formation with reduced ferredoxin Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + electron acceptor methylene blue as electron acceptor Methanosarcina barkeri H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Methanosarcina barkeri H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + oxidized ferredoxin
-
 Methanosarcina barkeri H+ + reduced ferredoxin
-
 r
1.12.7.2 additional information major membrane protein in acetate-grown, methanol-grown or H2/CO2-grown cells Methanosarcina barkeri ?
-
 ?
1.12.7.2 additional information major membrane protein in acetate-grown, methanol-grown or H2/CO2-grown cells Methanosarcina barkeri Fusaro / DSM 804 ?
-
 ?
1.12.98.3 H2 + 2-(2,3-dihydropentaprenyloxy)phenazine no activity with ferredoxin Methanosarcina barkeri 2-dihydropentaprenyloxyphenazine
-
 ?
1.12.99.6 H2 + acceptor benzyl viologen, methylene blue, ferredoxin and methylviologen can act as electron acceptors Methanosarcina barkeri H+ + reduced acceptor
-
 ?
1.12.99.6 H2 + acceptor ferredoxin acts as physiological electron donor Methanosarcina barkeri H+ + reduced acceptor
-
 ?
1.12.99.6 H2 + acceptor benzyl viologen, methylene blue, ferredoxin and methylviologen can act as electron acceptors Methanosarcina barkeri Fusaro / DSM 804 H+ + reduced acceptor
-
 ?
1.12.99.6 H2 + acceptor ferredoxin acts as physiological electron donor Methanosarcina barkeri Fusaro / DSM 804 H+ + reduced acceptor
-
 ?
1.12.99.6 H2 + oxidized ferredoxin
-
 Methanosarcina barkeri reduced ferredoxin
-
 r
1.12.99.6 H2 + oxidized ferredoxin
-
 Methanosarcina barkeri Fusaro / DSM 804 reduced ferredoxin
-
 r
1.12.99.6 reduced ferredoxin
-
 Methanosarcina barkeri H2 + oxidized ferredoxin
-
 r
1.12.99.6 reduced ferredoxin
-
 Methanosarcina barkeri Fusaro / DSM 804 H2 + oxidized ferredoxin
-
 r

Subunits

EC Number Subunits Comment Organism
1.12.98.3 ? x * 57000 + x * 35000, SDS-PAGE Methanosarcina barkeri
1.12.99.6 hexamer 6 different subunits A, B, C, D, E, and F, SDS-PAGE Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.99.6 7
-
-
 Methanosarcina barkeri